CAJ | 학술논문

目的：研究复脉汤对急性心肌缺血大鼠肌钙蛋白Ⅰ（cTnⅠ）含量及心肌组织超微结构的影响。方法：将SD大鼠分为假手术组、模型组、炙甘草汤组、酒石酸美托洛尔组、复脉汤高、中、低剂量组，灌胃给药14 d后，结扎SD大鼠冠状动脉左前降支维持1 h（除假手术组外），制成急性心肌缺血模型，腹主动脉取血采用ELISA法检测血清cTnⅠ含量，电镜下观察各组大鼠心肌细胞的形态改变。结果：各剂量复脉汤、美托洛尔可不同程度降低cTnI浓度，减轻心肌缺血导致的心肌细胞超微结构的损伤。与炙甘草汤比较，差异有统计学意义（P＜0.05，P＜0.01）。结论：复脉汤对结扎左冠状动脉造成的急性心肌缺血大鼠具有保护作用，其作用机制与降低外周血cTnⅠ水平及改善心肌超微结构损伤有关。
목적：연구복맥탕대급성심기결혈대서기개단백Ⅰ（cTnⅠ）함량급심기조직초미결구적영향。방법：장SD대서분위가수술조、모형조、자감초탕조、주석산미탁락이조、복맥탕고、중、저제량조，관위급약14 d후，결찰SD대서관상동맥좌전강지유지1 h（제가수술조외），제성급성심기결혈모형，복주동맥취혈채용ELISA법검측혈청cTnⅠ함량，전경하관찰각조대서심기세포적형태개변。결과：각제량복맥탕、미탁락이가불동정도강저cTnI농도，감경심기결혈도치적심기세포초미결구적손상。여자감초탕비교，차이유통계학의의（P＜0.05，P＜0.01）。결론：복맥탕대결찰좌관상동맥조성적급성심기결혈대서구유보호작용，기작용궤제여강저외주혈cTnⅠ수평급개선심기초미결구손상유관。
Objective:To study the effect of Fumai decoction on serum cardiac troponinⅠ(cTnⅠ)content and ultrastructure of cardiac muscle of rat models with acute myocardial ischemia. Methods:SD rats were randomly divided into sham opera-tion group,model group,Zhigancao decoction group,metoprolol tartrate group,high,medium and low dosage groups of Fu-mai decoction. 14 days after the administration,rat models of acute myocardial ischemia were prepared,then concentration of cTnI in the serum of rats was detected by ELISA. Morphological changes of myocardial cells were observed under elec-tron microscope. Results:Compared with Zhigancao decoction group,concentration of cTnⅠ was decreased and injury of myocardial cell was reduced in high and medium dosage groups of Fumai decoction and metoprolol tartrate group(P<0.05, P<0.01). Conclusion:Fumai decoction had protective effect on rats with acute myocardial ischemia through reducing cTnⅠlevel in the peripheral blood and improving myocardial ultrastructure.