CAJ | 학술논문

蒎烯是一种重要的平台化合物，可以用来合成高密度燃料、精细化学品及材料。将来源于黄花蒿的β蒎烯合成酶基因与外源杂合甲羟戊酸（ MVA）途径一起整合到BL21（ DE3）中共表达，通过气相色谱质谱（ GC MS）和气相色谱（ GC）对发酵产物进行了定性及定量检测。通过发酵条件优化，对影响发酵产β蒎烯的因素（诱导温度、诱导剂浓度和N源）进行了考察。结果表明：来自黄花蒿的蒎烯合酶可以在宿主细胞内高效表达，且能高效催化β蒎烯的合成。通过对重组菌进行发酵条件优化得到最佳培养条件：诱导温度为28℃，IPTG 浓度为0?1 mmol／L，最佳有机N源为MD牛肉粉。在此条件下，检测出β蒎烯产量达到22?89 mg／L，比优化前（4?60 mg／L）提高了3?98倍。
파희시일충중요적평태화합물，가이용래합성고밀도연료、정세화학품급재료。장래원우황화호적β파희합성매기인여외원잡합갑간무산（ MVA）도경일기정합도BL21（ DE3）중공표체，통과기상색보질보（ GC MS）화기상색보（ GC）대발효산물진행료정성급정량검측。통과발효조건우화，대영향발효산β파희적인소（유도온도、유도제농도화N원）진행료고찰。결과표명：래자황화호적파희합매가이재숙주세포내고효표체，차능고효최화β파희적합성。통과대중조균진행발효조건우화득도최가배양조건：유도온도위28℃，IPTG 농도위0?1 mmol／L，최가유궤N원위MD우육분。재차조건하，검측출β파희산량체도22?89 mg／L，비우화전（4?60 mg／L）제고료3?98배。
Pinene is an important natural product that is widely used in high?density renewable fuels and fine chemicals. The Artemisia annua β?pinene synthase gene ( QH6 ) was successfully assembled in Escherichia coli BL21 ( DE3 ) with the heterologous hybrid mevalonate ( MVA ) pathway. Pinene was quantitatively analyzed and characterized by gas chromatography( GC) and GC?mass spectrometry( MS) . The influences of induction temperature, inducer concentration, and nitrogen on production of β?pinene were studied. After shake?flask fermentation,β?pinene productivity of the engineered strain was 4?60 mg/L. Under the optimum conditions of induction temperature 28 ℃,inducer ( IPTG) concentration 0?1 mmol/L,and beef extract powder(MDbio Inc?),the β?pinene yield was up to 22?89 mg/L,3?98 times higher than that before optimization.