CAJ | 학술논문

目的：探讨电针、埋线对吗啡成瘾小鼠大脑视交叉上核（suprachiasmatic nucleus，SCN）内生物钟基因Per1、Per2表达的影响。方法：筛选雄性 C57BL/6J近交系小鼠42只，随机分为空白组（n=14）、模型组（n=14）、电针组（n=7）、埋线组（n=7）。动物模型根据“7 d递增成瘾法”皮下注射盐酸吗啡注射液。第7天取小鼠SCN，采用实时荧光定量PCR法检测Per1、Per2基因的表达水平。结果：与空白组小鼠比较，模型组小鼠SCN内Per1、Per2基因表达均下调，差异具有统计学意义（P＜0.05）；与模型组小鼠比较，电针组Per2基因表达明显上调（P＜0.05），埋线组Per1基因表达明显上调（P＜0.05）；电针组与埋线组比较，差异无统计学意义（P＞0.05）。结论：埋线和电针可以分别调节Per1、Per2基因的表达，从而改善吗啡成瘾小鼠紊乱的节律。
목적：탐토전침、매선대마배성은소서대뇌시교차상핵（suprachiasmatic nucleus，SCN）내생물종기인Per1、Per2표체적영향。방법：사선웅성 C57BL/6J근교계소서42지，수궤분위공백조（n=14）、모형조（n=14）、전침조（n=7）、매선조（n=7）。동물모형근거“7 d체증성은법”피하주사염산마배주사액。제7천취소서SCN，채용실시형광정량PCR법검측Per1、Per2기인적표체수평。결과：여공백조소서비교，모형조소서SCN내Per1、Per2기인표체균하조，차이구유통계학의의（P＜0.05）；여모형조소서비교，전침조Per2기인표체명현상조（P＜0.05），매선조Per1기인표체명현상조（P＜0.05）；전침조여매선조비교，차이무통계학의의（P＞0.05）。결론：매선화전침가이분별조절Per1、Per2기인적표체，종이개선마배성은소서문란적절률。
Objective:To discuss the effects of electro-acupuncture and catgut embedding in regulating expressions of Per1 and Per2 in suprachiasmatic nucleus(SCN) of morphine-addiction mice. Methods:42 male C57BL/6J mice were ran-domly divided into blank group(n=14),model group(n=14),electro-acupuncture group(n=7)and catgut embedding group (n=7). Models were induced by injecting morphine hydrochloride injection. Samples of SCN were removed and Per1 and Per2 genes expression were measured by RT-PCR method. Results:Compared with blank group,gene expressions of Per2 and Per1 were up-regulated in model group (P<0.05). Compared with model group,Per1 expression of catgut embedding group and Per2 expression of electro-acupuncture group were up-regulated(P<0.05). Conclusion:Therapies of catgut embedding and electro-acupuncture could improve indiscriminate rhythm of morphine dependent mice through regulating expressions of Per1 and Per2.