CAJ | 학술논문

目的：筛选铁皮石斛组培苗生根壮苗的适宜培养基。方法在相同的培养条件下，将铁皮石斛相同高度幼苗接种于9种不同的培养基中，记录各组组培苗的生长情况；采用紫外分光光度法测定各组组培苗的多糖含量并进行综合分析。结果铁皮石斛组培苗的生根壮苗以1/2-MS+土豆泥+0.5-mg-1·L7BA+0.2-mg-1·L-NAA+活性炭+花宝1号+3%蔗糖或1/2-MS+土豆泥+0.3-mg-1·L-BA+0.15-mg-1·L-NAA+活性炭+花宝1号+3%蔗糖的培养基为佳。
목적：사선철피석곡조배묘생근장묘적괄의배양기。방법재상동적배양조건하，장철피석곡상동고도유묘접충우9충불동적배양기중，기록각조조배묘적생장정황；채용자외분광광도법측정각조조배묘적다당함량병진행종합분석。결과철피석곡조배묘적생근장묘이1/2-MS+토두니+0.5-mg-1·L7BA+0.2-mg-1·L-NAA+활성탄+화보1호+3%자당혹1/2-MS+토두니+0.3-mg-1·L-BA+0.15-mg-1·L-NAA+활성탄+화보1호+3%자당적배양기위가。
to optimize the most appropriate culture medium for rooting and growth-promoting of Dendrobium officinale seedlings. in the same culture conditions, the same height seedlings were seeded in 9 different medium, compared the growth of the seedlings through the observation records; used UV spectrophotometry to de tect polysaccharide content of each cultivation and made the comprehensive analysis. Results and Conclusion: the best medium for rooting and growth-promoting of Dendrobium officinale Seedlings was 1/2 MS + mashed potatoes + 0.5 mg/L BA + 0.2 mg/L NAA + activated carbon + Huabao NO.1 + 3%sucrose,or, 1/2,MS,+,mashed,potatoes,+,0.3,mg/L,BA,+,0.15,mg/L,NAA,+,activated,carbon,+,Huabao,NO.1,+,3%,sucrose.