CAJ | 학술논문

以荞麦‘右玉26’为材料，在防雨棚下进行盆栽试验，研究外源硒对土壤铅污染下荞麦幼苗的农艺性状及生理特性、成熟期各器官铅累积和产量的影响，旨在探讨硒对土壤铅胁迫下荞麦的生长发育、产量及铅在荞麦体内富集量的影响，为发现硒缓解重金属铅对植物的毒害作用提供理论依据，以期为农业区铅污染治理及荞麦生产提供有价值的思路和方法。试验采用2因素完全随机设计，设置3个土壤铅浓度(0 mg·L?1、500 mg·L?1、1000 mg·L?1)、5个硒浓度(0 mg·kg?1、1 mg·kg?1、2.5 mg·kg?1、5 mg·kg?1和10 mg·kg?1)。研究结果表明：1)随铅浓度增加，荞麦的株高、总根长、干重、根系总面积、根系活力、叶绿素含量、叶绿素荧光参数、经济产量(千粒重、株粒数)均呈降低趋势。2)低浓度硒(1~2.5 mg·L?1)可缓解铅对荞麦的毒害，高浓度硒(5~10 mg·L?1)和铅为协同作用，加剧了对荞麦的毒害。3)硒浓度为2.5 mg·L?1时，缓解铅对荞麦毒害的效果最为明显，显著降低了铅胁迫下荞麦幼苗SOD和POD活性；降低成熟期荞麦各器官铅含量，各处理下荞麦各器官铅含量均表现为根>茎>叶>种子；产量达到最大值。由此可见，外源硒可通过抑制荞麦对铅的吸收和转运，促进荞麦幼苗叶片的光合作用、提高叶片叶绿素含量和根系活力等途径来增强荞麦对铅胁迫的耐性。对本研究所用的5种浓度来说，缓解效果最佳的硒浓度为2.5 mg·L?1。
이교맥‘우옥26’위재료，재방우붕하진행분재시험，연구외원서대토양연오염하교맥유묘적농예성상급생리특성、성숙기각기관연루적화산량적영향，지재탐토서대토양연협박하교맥적생장발육、산량급연재교맥체내부집량적영향，위발현서완해중금속연대식물적독해작용제공이론의거，이기위농업구연오염치리급교맥생산제공유개치적사로화방법。시험채용2인소완전수궤설계，설치3개토양연농도(0 mg·L?1、500 mg·L?1、1000 mg·L?1)、5개서농도(0 mg·kg?1、1 mg·kg?1、2.5 mg·kg?1、5 mg·kg?1화10 mg·kg?1)。연구결과표명：1)수연농도증가，교맥적주고、총근장、간중、근계총면적、근계활력、협록소함량、협록소형광삼수、경제산량(천립중、주립수)균정강저추세。2)저농도서(1~2.5 mg·L?1)가완해연대교맥적독해，고농도서(5~10 mg·L?1)화연위협동작용，가극료대교맥적독해。3)서농도위2.5 mg·L?1시，완해연대교맥독해적효과최위명현，현저강저료연협박하교맥유묘SOD화POD활성；강저성숙기교맥각기관연함량，각처리하교맥각기관연함량균표현위근>경>협>충자；산량체도최대치。유차가견，외원서가통과억제교맥대연적흡수화전운，촉진교맥유묘협편적광합작용、제고협편협록소함량화근계활력등도경래증강교맥대연협박적내성。대본연구소용적5충농도래설，완해효과최가적서농도위2.5 mg·L?1。
This study used ‘Youyu 26’ buckwheat cultivar to analyze the effects of exogenous selenium (Se) on agronomic and physiological characteristics of buckwheat seedlings in plumbum (Pb) polluted soil in a pot experiment. The study determined the effects of exogenous Se on Pb content, and growth, development and output of buckwheat under soil Pb stress. The results of the study could lay the theory base for research on the use of Se to alleviate the toxicity of heavy metal Pb in plants. This was critical for developing the methodology needed for producing buckwheat and decreasing heavy mental contamination in agricultural fields. The experiment was set up in a two-factor complete random design, with five concentrations of Se (0 mg·kg?1, 1 mg·kg?1, 2.5 mg·kg?1, 5 mg·kg?1 and 10 mg·kg?1) and three concentrations of Pb (0 mg·L?1, 500 mg·L?1 and 1 000 mg·L?1). The results showed that with increasing concentration of Pb, plant height, total root length, dry weight, total root area, root activity, chlorophyll content, chlorophyll fluorescence parameters and economic yield (1000-grain weight, per-plant grain, etc.) of buckwheat decreased. Low Se concentration (1?2.5 mg·L?1) alleviated Pb toxicity in buckwheat. The synergistic effect of high concentration of Se (5?10 mg·L?1) increased Pb toxicity in buckwheat. Buckwheat total root length, root area, root activity and photosynthetic characteristics were highest and SOD and POD activities lowest at Se concentration of 2.5 mg·L?1. Economic yield of buckwheat was highest when Pb tolerance of all organs of mature buckwheat was lowest at Se concentration of 2.5 mg·L?1. Under the same Pb concentration, the Pb <br> content in organs of buckwheat was decreased first and then increased with increased Se concentration. The Pb contents in different organs of buckwheat followed the order of root> stem > leaf> seeds under each treatment. Thus exogenous Se could be used to enhance buckwheat tolerance to Pb stress by inhabiting buckwheat absorption and transportation of Pb, promoting leaf photosynthesis and chlorophyll content along with root activity of seedling buckwheat. The research showed that suitable amount of Se (a recommended concentration of 2.5 mg·L?1) could alleviate heavy metal toxicity in buckwheat.