中国当代儿科杂志
中國噹代兒科雜誌
중국당대인과잡지
CHINA JOURNAL OF CONTEMPORARY PEDIATRICS
2015年
4期
312-316
,共5页
喘息%髓源性抑制细胞%Th17细胞%婴幼儿
喘息%髓源性抑製細胞%Th17細胞%嬰幼兒
천식%수원성억제세포%Th17세포%영유인
Wheezing%Myeloid-derived suppressor cell%T-helper17 cell%Young child
目的：探讨反复喘息婴幼儿外周血髓源性抑制细胞（MDSC）及Th17细胞比例及临床意义。方法随机选取急性发作期的反复喘息婴幼儿30例作为喘息组，同时选取同年龄段20例支气管肺炎患儿作为肺炎组，另选取同年龄段在该院外科住院的疝气、肾结石等非感染性、非肿瘤性疾病术前患儿23例作为对照组。流式细胞术检测各组患儿外周血中MDSC、Th17细胞的比例，采用Spearman相关性分析MDSC及Th17细胞比例的相关性。结果喘息组患儿MDSC占有核细胞的百分比明显高于肺炎组和对照组，肺炎组高于对照组（P<0.05）。喘息组Th17细胞占单个核细胞的百分比明显高于肺炎组及对照组，肺炎组与对照组Th17比较差异无统计学意义。喘息组外周血MDSC及Th17细胞比例呈正相关关系（r=0.645，P<0.01）。结论 MDSC及Th17细胞可能参与了婴幼儿反复喘息的发生发展。
目的：探討反複喘息嬰幼兒外週血髓源性抑製細胞（MDSC）及Th17細胞比例及臨床意義。方法隨機選取急性髮作期的反複喘息嬰幼兒30例作為喘息組，同時選取同年齡段20例支氣管肺炎患兒作為肺炎組，另選取同年齡段在該院外科住院的疝氣、腎結石等非感染性、非腫瘤性疾病術前患兒23例作為對照組。流式細胞術檢測各組患兒外週血中MDSC、Th17細胞的比例，採用Spearman相關性分析MDSC及Th17細胞比例的相關性。結果喘息組患兒MDSC佔有覈細胞的百分比明顯高于肺炎組和對照組，肺炎組高于對照組（P<0.05）。喘息組Th17細胞佔單箇覈細胞的百分比明顯高于肺炎組及對照組，肺炎組與對照組Th17比較差異無統計學意義。喘息組外週血MDSC及Th17細胞比例呈正相關關繫（r=0.645，P<0.01）。結論 MDSC及Th17細胞可能參與瞭嬰幼兒反複喘息的髮生髮展。
목적：탐토반복천식영유인외주혈수원성억제세포（MDSC）급Th17세포비례급림상의의。방법수궤선취급성발작기적반복천식영유인30례작위천식조，동시선취동년령단20례지기관폐염환인작위폐염조，령선취동년령단재해원외과주원적산기、신결석등비감염성、비종류성질병술전환인23례작위대조조。류식세포술검측각조환인외주혈중MDSC、Th17세포적비례，채용Spearman상관성분석MDSC급Th17세포비례적상관성。결과천식조환인MDSC점유핵세포적백분비명현고우폐염조화대조조，폐염조고우대조조（P<0.05）。천식조Th17세포점단개핵세포적백분비명현고우폐염조급대조조，폐염조여대조조Th17비교차이무통계학의의。천식조외주혈MDSC급Th17세포비례정정상관관계（r=0.645，P<0.01）。결론 MDSC급Th17세포가능삼여료영유인반복천식적발생발전。
ObjectiveTo determine the frequenciesand signiifcance of myeloid-derived suppressor cells (MDSCs) and T-helper 17 (Th17) cells in peripheral blood of young children with recurrent wheezing.MethodsThirty young children with an acute exacerbation of recurrent wheezing were randomly enrolled. Twenty age-matched children with bronchopneumonia (pneumonia group) and 23 age-matched preoperative children with non-infectious or non-neoplastic diseases (hernia or renal calculus) (control group) were selected. The frequencies of MDSCs and Th17 cells in the peripheral blood were measured using lfow cytometry and their correlation was determined by the Spearman’s correlation coefifcient.ResultsThe percentage of MDSCs in nucleated cells was signiifcantly higher in the wheezing group than in the pneumonia and control groups (P<0.05), and it was significantly higher in the pneumonia group than in the control group (P<0.05). The percentage of Th17 cells in mononuclear cells was signiifcantly higher in the wheezing group than in the pneumonia and control groups (P<0.05), but it showed no signiifcant difference between the pneumonia and control groups (P>0.05). The frequency of MDSCs was positively correlated with the frequency of Th17 cells in the wheezing group (r=0.645,P<0.01).ConclusionsMDSCs and Th17 cells may contribute to the pathogenesis of recurrent wheezing in young children.
