CAJ | 학술논문

目的：探讨辅助性T细胞9（Th9）及其细胞因子白细胞介素-9（IL-9）在肺炎支原体（MP）感染患儿中的临床意义。方法2013年1月至2014年6月诊断为MP感染患儿86例，分为上呼吸道感染组（URI组， n=29）、轻症MP肺炎（MPP）组（n=32）和重症MPP组（n=25）。另选取28例同期行健康体检儿童为对照组。Ficoll密度梯度离心法分离外周血单个核细胞，采用流式细胞术检测各组外周血Th9细胞百分率；ELISA法检测各组血清细胞因子IL-9水平。结果 URI组、轻症MPP组和重症MPP组患儿外周血Th9细胞百分率及IL-9水平均高于对照组（P<0.05）；轻症MPP组和重症MPP组患儿外周血Th9细胞百分率及IL-9水平均高于URI组（P<0.05），且重症MPP组显著高于轻症MPP组（P<0.01）。结论 MP感染患儿外周血Th9细胞百分率及其细胞因子IL-9的表达水平升高，且随病情加重而增加，推测Th9细胞及IL-9可作为MP感染患儿病情发展及转归的评价指标。
목적：탐토보조성T세포9（Th9）급기세포인자백세포개소-9（IL-9）재폐염지원체（MP）감염환인중적림상의의。방법2013년1월지2014년6월진단위MP감염환인86례，분위상호흡도감염조（URI조， n=29）、경증MP폐염（MPP）조（n=32）화중증MPP조（n=25）。령선취28례동기행건강체검인동위대조조。Ficoll밀도제도리심법분리외주혈단개핵세포，채용류식세포술검측각조외주혈Th9세포백분솔；ELISA법검측각조혈청세포인자IL-9수평。결과 URI조、경증MPP조화중증MPP조환인외주혈Th9세포백분솔급IL-9수평균고우대조조（P<0.05）；경증MPP조화중증MPP조환인외주혈Th9세포백분솔급IL-9수평균고우URI조（P<0.05），차중증MPP조현저고우경증MPP조（P<0.01）。결론 MP감염환인외주혈Th9세포백분솔급기세포인자IL-9적표체수평승고，차수병정가중이증가，추측Th9세포급IL-9가작위MP감염환인병정발전급전귀적평개지표。
ObjectiveTo investigate the clinical signiifcance of T helper type 9 (Th9) cells and interleukin-9 (IL-9) in children suffering from Mycoplasma pneumoniae (MP) infection.MethodsA total of 86 children who were diagnosed with MP infection between January 2013 and June 2014 were classified into upper respiratory infection (URI) group (n=29), mild MP pneumonia (MPP) group (n=32) and severe MPP group (n=25). Twenty-eight healthy children were used as the control group. Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation, and the percentage of Th9 cells in peripheral blood was measured by flow cytometry. Serum IL-9 level was determined using ELISA.ResultsThe URI, mild MPP, and severe MPP groups had signiifcantly higher percentages of Th9 cells and IL-9 levels than the control group (P<0.05); the mild MPP and severe MPP groups had signiifcantly higher percentages of Th9 cells and IL-9 levels than the URI group (P<0.05), and the two indices were signiifcantly higher in the severe MPP group than in the mild MPP group (P<0.01).ConclusionsChildren with MP infection have an elevated percentage of Th9 cells and IL-9 expression, both of which are positively correlated with the severity of the disease. It can be predicted that Th9 cells and IL-9 can be used as evaluation indicators for the progression and outcome of children with MP infection.