中国当代儿科杂志
中國噹代兒科雜誌
중국당대인과잡지
CHINA JOURNAL OF CONTEMPORARY PEDIATRICS
2015年
4期
332-336
,共5页
陈小文%飞球%吕荣钰%张民%祖莹%麦惠容%王缨%袁秀丽%李长钢%马东礼
陳小文%飛毬%呂榮鈺%張民%祖瑩%麥惠容%王纓%袁秀麗%李長鋼%馬東禮
진소문%비구%려영옥%장민%조형%맥혜용%왕영%원수려%리장강%마동례
急性淋巴细胞白血病%融合基因%多重逆转录聚合酶链式反应%儿童
急性淋巴細胞白血病%融閤基因%多重逆轉錄聚閤酶鏈式反應%兒童
급성림파세포백혈병%융합기인%다중역전록취합매련식반응%인동
Acute lymphoblastic leukemia%Fusion transcript%Multiplex RT-PCR%Child
目的：探讨一步法多重RT-PCR对儿童急性淋巴细胞白血病（ALL）常见4种融合基因的检测效果。方法2003年1月至2010年12月确诊的ALL患儿76例，采集所有患儿骨髓标本，提取细胞总RNA，一步法多重RT-PCR或常规巢式PCR法对E2A/PBX1、MLL/AF4、BCR/ABL和TEL/AML1融合基因进行检测；DNA测序对PCR结果进行验证。结果一步法多重RT-PCR及DNA测序验证显示76例ALL患儿中，TEL/AML1融合基因阳性12例（产物长度分别为298 bp 9例，259 bp 3例）， E2A/PBX1融合基因阳性3例（产物长度373 bp）， BCR/ABL融合基因阳性1例（产物长度2124 bp），MLL/AF4融合基因阳性7例（产物长度分别为427 bp 1例，673 bp 6例），与常规巢式PCR检出结果一致。结论一步法多重RT-PCR可应用于儿童ALL常见融合基因的检测。
目的：探討一步法多重RT-PCR對兒童急性淋巴細胞白血病（ALL）常見4種融閤基因的檢測效果。方法2003年1月至2010年12月確診的ALL患兒76例，採集所有患兒骨髓標本，提取細胞總RNA，一步法多重RT-PCR或常規巢式PCR法對E2A/PBX1、MLL/AF4、BCR/ABL和TEL/AML1融閤基因進行檢測；DNA測序對PCR結果進行驗證。結果一步法多重RT-PCR及DNA測序驗證顯示76例ALL患兒中，TEL/AML1融閤基因暘性12例（產物長度分彆為298 bp 9例，259 bp 3例）， E2A/PBX1融閤基因暘性3例（產物長度373 bp）， BCR/ABL融閤基因暘性1例（產物長度2124 bp），MLL/AF4融閤基因暘性7例（產物長度分彆為427 bp 1例，673 bp 6例），與常規巢式PCR檢齣結果一緻。結論一步法多重RT-PCR可應用于兒童ALL常見融閤基因的檢測。
목적：탐토일보법다중RT-PCR대인동급성림파세포백혈병（ALL）상견4충융합기인적검측효과。방법2003년1월지2010년12월학진적ALL환인76례，채집소유환인골수표본，제취세포총RNA，일보법다중RT-PCR혹상규소식PCR법대E2A/PBX1、MLL/AF4、BCR/ABL화TEL/AML1융합기인진행검측；DNA측서대PCR결과진행험증。결과일보법다중RT-PCR급DNA측서험증현시76례ALL환인중，TEL/AML1융합기인양성12례（산물장도분별위298 bp 9례，259 bp 3례）， E2A/PBX1융합기인양성3례（산물장도373 bp）， BCR/ABL융합기인양성1례（산물장도2124 bp），MLL/AF4융합기인양성7례（산물장도분별위427 bp 1례，673 bp 6례），여상규소식PCR검출결과일치。결론일보법다중RT-PCR가응용우인동ALL상견융합기인적검측。
ObjectiveTo evaluate the efficiency of one-step multiplex RT-PCR for identifying four common fusion transcripts (TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL) in children with acute lymphoblastic leukemia (ALL).MethodsTotal RNA was extracted from bone marrow samples of 76 children who were newly diagnosed with ALL between January 2003 and December 2010. These RNAs were analyzed for TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL by one-step multiplex RT-PCR or common nested-multiplex PCR. The PCR products were conifrmed by DNA sequencing.ResultsTEL/AML1 was found in 12 cases (the length of products was 298 bp in 9 cases and 259 bp in 3 cases), E2A/PBX1 was found in 3 cases (the length of products was 373 bp), BCR/ABL was found in 1 case (the length of products was 2 124 bp), and MLL/AF4 was found in 7 cases (the length of products was 427 bp in 1 case and 673 bp in 6 cases) using one-step multiplex RT-PCR combined with DNA sequencing. The results were consistent with those using common nested-multiplex PCR.ConclusionsOne-step multiplex RT-PCR may be another alternative for detection of common fusion transcripts in children with ALL.
