CAJ | 학술논문

目的探讨肺表面活性蛋白A2(SP-A2)单体型在中国局部地区人群的分布及其与早产儿呼吸窘迫综合征(RDS)发病的关系.方法采用人群研究和病例对照研究方法,选取RDS早产儿50例(RDS组)及性别相同、胎龄接近的非RDS早产儿50例(对照组)为研究对象,同时以80例足月分娩的健康足月儿为人群研究对象.采用TaqMan(R)实时荧光定量聚合酶链反应法对SP-A2基因的4个单核甘酸多态性位点(rs1059046、rs17886395、rs1965707、rs1965708)进行基因分型,然后进行单体型分析,并在RDS组和对照组、对照组和足月儿组之间进行单体型频率的比较.结果足月儿主要表达的单体型为1A0、1A5、1A1.早产儿RDS组和对照组中也是以1A0、1A5、1A1为主要表达的单体型,但2组中1A0频率均较足月儿低.在RDS组和对照组间,各单体型频率比较差异无统计学意义(P＞0.05),即使在不同的胎龄分组中(胎龄≥32周或＜32周),各单体型频率在RDS组和对照组间比较差异也无统计学意义.足月儿中1A0频率(0.542)与＜32周对照组(0.329)相比,差异有统计学意义(x2=6.06,P=0.01).结论在中国南方局部汉族人群中SP-A2的单体型分布有一定特异性.未发现各单体型与早产儿RDS发病存在关联.
목적 탐토폐표면활성단백A2(SP-A2)단체형재중국국부지구인군적분포급기여조산인호흡군박종합정(RDS)발병적관계.방법 채용인군연구화병례대조연구방법,선취RDS조산인50례(RDS조)급성별상동、태령접근적비RDS조산인50례(대조조)위연구대상,동시이80례족월분면적건강족월인위인군연구대상.채용TaqMan(R)실시형광정량취합매련반응법대SP-A2기인적4개단핵감산다태성위점(rs1059046、rs17886395、rs1965707、rs1965708)진행기인분형,연후진행단체형분석,병재RDS조화대조조、대조조화족월인조지간진행단체형빈솔적비교.결과 족월인주요표체적단체형위1A0、1A5、1A1.조산인RDS조화대조조중야시이1A0、1A5、1A1위주요표체적단체형,단2조중1A0빈솔균교족월인저.재RDS조화대조조간,각단체형빈솔비교차이무통계학의의(P＞0.05),즉사재불동적태령분조중(태령≥32주혹＜32주),각단체형빈솔재RDS조화대조조간비교차이야무통계학의의.족월인중1A0빈솔(0.542)여＜32주대조조(0.329)상비,차이유통계학의의(x2=6.06,P=0.01).결론 재중국남방국부한족인군중SP-A2적단체형분포유일정특이성.미발현각단체형여조산인RDS발병존재관련.
Objective To study the distribution of surfactant protein A2 (SP-A2) haplotype and its association with preterm respiratory distress syndrome (RDS) susceptibility in a local Chinese cohort.Methods Using population base and case-control study cohorts,genotyping for four single nucleotide polymorphism (SNP) was performed,rs1059046,rs17886395,rs1965707,rs1965708,in 80 term infants,50 preterm infants with RDS (RDS group) and 50 preterm infants without RDS(control group) by using TaqMan (R) real-time polymerase chain reaction.Infants in RDS group and control group were matched according to gender and gestational age.Frequency of each haplotype was compared between preterm infants with RDS and without RDS,term infants and preterm infants without RDS.Results Most common haplotypes in term infants were 1A0,1A5,1A1.In each preterm infants groups with RDS and without RDS,1A0,1 A5,1 A1 were also the most common haplotypes.Among these three common haplotypes,frequency of 1A0 was lower in preterm infants,including RDS group and control group,than that in term infants.No significant difference was found between preterm groups with RDS and without RDS (P ＞ 0.05),neither in preterm infants with gestational age ≥32 or ＜ 32 weeks.Haplotype 1A0 frequency(0.542) in term infants was significantly higher than that in preterm infants ＜ 32 weeks without RDS (0.329) (x2 =6.06,P =0.01).Conclusions SP-A2 haplotype distribution in a local Chinese population shows ethnic characteristics to some extent.Only SP-A2 does not contribute to the susceptibility for preterm RDS.