中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2015年
4期
72-74
,共3页
贾宇旻%吴晓婵%渠光辉%黎文娟%李晓云
賈宇旻%吳曉嬋%渠光輝%黎文娟%李曉雲
가우민%오효선%거광휘%려문연%리효운
亚硝酸盐%快速检测%检测试纸条%分光光度法
亞硝痠鹽%快速檢測%檢測試紙條%分光光度法
아초산염%쾌속검측%검측시지조%분광광도법
nitrites%rapid detection%test strip%spectrophotometry
利用与对氨基苯磺酸和盐酸萘乙二胺反应显色的原理,探索最佳的试纸卡制作工艺,将对氨基苯磺酸和盐酸萘乙二胺分别浸在试纸上,干燥,组装成亚硝酸盐速测试纸卡。同时制备含量依次为100 mg/L、80 mg/L、60 mg/L、40 mg/L、20 mg/L、10 mg/L、5.0mg/L、2mg/L、1mg/L的亚硝酸盐溶液制成标准比色卡,作为比色对照。经与分光光度法做了灵敏度和准确度的对比,该方法具有快速、简便、灵敏度和准确度高等特点。
利用與對氨基苯磺痠和鹽痠萘乙二胺反應顯色的原理,探索最佳的試紙卡製作工藝,將對氨基苯磺痠和鹽痠萘乙二胺分彆浸在試紙上,榦燥,組裝成亞硝痠鹽速測試紙卡。同時製備含量依次為100 mg/L、80 mg/L、60 mg/L、40 mg/L、20 mg/L、10 mg/L、5.0mg/L、2mg/L、1mg/L的亞硝痠鹽溶液製成標準比色卡,作為比色對照。經與分光光度法做瞭靈敏度和準確度的對比,該方法具有快速、簡便、靈敏度和準確度高等特點。
이용여대안기분광산화염산내을이알반응현색적원리,탐색최가적시지잡제작공예,장대안기분광산화염산내을이알분별침재시지상,간조,조장성아초산염속측시지잡。동시제비함량의차위100 mg/L、80 mg/L、60 mg/L、40 mg/L、20 mg/L、10 mg/L、5.0mg/L、2mg/L、1mg/L적아초산염용액제성표준비색잡,작위비색대조。경여분광광도법주료령민도화준학도적대비,해방법구유쾌속、간편、령민도화준학도고등특점。
The best process for preparation of test strips for rapid detection of nitritesin food was explored by using the color reaction principle of sulphanilic acid and naphthyl ethylenediamine dihydrochloride. The sulphanilic acid and naphthyl ethylenediamine dihydrochloride were respectively soaked on to the test paper and the test paper was assembled to rapid test strips when dried. Meanwhile,reference colorimetric card was prepared with 100 mg/L,80 mg/L, 60 mg/L,40 mg/L,20 mg/L,10 mg/L,5.0mg/L 2mg/L,1mg/L nitrites as colorimetric control. The method was rapid,simple,sensitive and accurate as compared with spectrophotometry.