CAJ | 학술논문

田间试验条件下，为了探究非抗虫转基因棉花对土壤细菌群落多样性的影响，应用PCR-DGGE技术对转RRM2基因高产棉、转GAFP基因抗病棉、转ACO2基因优质棉及非转基因常规棉（中棉所12）种植后在吐絮期的土壤细菌群落多样性进行分析。结果表明，与常规棉相比，3种转基因棉的种植均未对土壤细菌香农-威纳指数（H）、均匀度（EH）和丰富度（S）造成显著影响，且两类棉花土壤细菌群落结构相似性较高。可见，短期内，非抗虫转基因棉花的种植对土壤细菌群落多样性无显著影响。对DGGE的优势条带序列分析发现，同源性最高的微生物分别属于拟杆菌门（Bacteroidetes）的黄杆菌纲（Flavobacteria）、噬弧菌属（Bacteriovorax）、Segetibacter，变形菌门（Proteobacteria）的α-变形菌纲（alpha proteobacterium）、地杆菌属（Geobacter），厚壁菌门（Firmicutes）的Paenisporosarcina，酸杆菌门（Acidobacterias）的酸杆菌属（Acidobacterium），它们均为不可培养微生物。
전간시험조건하，위료탐구비항충전기인면화대토양세균군락다양성적영향，응용PCR-DGGE기술대전RRM2기인고산면、전GAFP기인항병면、전ACO2기인우질면급비전기인상규면（중면소12）충식후재토서기적토양세균군락다양성진행분석。결과표명，여상규면상비，3충전기인면적충식균미대토양세균향농-위납지수（H）、균균도（EH）화봉부도（S）조성현저영향，차량류면화토양세균군락결구상사성교고。가견，단기내，비항충전기인면화적충식대토양세균군락다양성무현저영향。대DGGE적우세조대서렬분석발현，동원성최고적미생물분별속우의간균문（Bacteroidetes）적황간균강（Flavobacteria）、서호균속（Bacteriovorax）、Segetibacter，변형균문（Proteobacteria）적α-변형균강（alpha proteobacterium）、지간균속（Geobacter），후벽균문（Firmicutes）적Paenisporosarcina，산간균문（Acidobacterias）적산간균속（Acidobacterium），타문균위불가배양미생물。
There are increasing public concerns over the ecological risks of transgenic plants. Under field conditions, the diversity and com-position of bacterial community in soils grown with three insect non-resistant transgenic cottons(high-yield transgenic cotton expressing the RNA recognition motif 2 gene, disease-resistant transgenic cotton expressing the gastrodia antifungal protein and high-quality transgenic cotton expressing the [1-aminocyclopropane-1-carboxylate(ACC)oxidase])and one conventional cotton CCRI 12(as control)were evalu-ated at the boll-opening stage by denaturing gradient gel electrophoresis(DGGE). The results showed that planting three transgenic cottons did not show significant effects on Shannon-wiener index(H), evenness(EH)and richness(S)of soil bacteria. High degree of similarity in community structure was observed between transgenic and conventional cottons, indicating no influence of transgenic cottons on bacterial community diversity in the short term. High-yield RRM2 transgenic cotton, disease-resistant GAFP transgenic cotton, high-quality ACO2 transgenic cotton and conventional cotton had 67%similarity in community levels and were thus regarded as one group. According to the se-quence analysis of DGGE dominant bands, microorganisms which presented the highest homology belonged to families of Flavobacteria, Bacteriovorax, Segetibacter, alpha proteobacterium, Geobacte, Paenisporosarcina, and Acidobacterium, respectively, and all of them were not cultivatable.