CAJ | 학술논문

目的：研究NHERF3在肾癌及其癌旁组织中的表达变化，为揭示肾癌组织NHERF3表达变化的临床意义提供线索。方法分别采用免疫组织化学法、Western blotting和RT-PCR方法检测肾癌及其对应癌旁组织中NHERF3蛋白及mRNA的表达，比较二者的差异。结果在癌旁组织中， NHERF3蛋白有较丰富的表达，而在肾癌组织中NHERF3蛋白的表达十分微弱( P<0.01)；癌旁组织NHERF3 mRNA表达水平亦明显高于肾癌组织(P<0.05)，二者之间的差异均有统计学意义。结论肾癌组织中NHERF3蛋白及mRNA与癌旁组织比较均呈现异常低表达，表明NHERF3基因表达在转录水平受到明显抑制，提示与肾癌NHERF3蛋白缺乏相关的NHERF3功能缺失( loss of function)可能在肾癌发生、发展中起重要作用。
목적：연구NHERF3재신암급기암방조직중적표체변화，위게시신암조직NHERF3표체변화적림상의의제공선색。방법분별채용면역조직화학법、Western blotting화RT-PCR방법검측신암급기대응암방조직중NHERF3단백급mRNA적표체，비교이자적차이。결과재암방조직중， NHERF3단백유교봉부적표체，이재신암조직중NHERF3단백적표체십분미약( P<0.01)；암방조직NHERF3 mRNA표체수평역명현고우신암조직(P<0.05)，이자지간적차이균유통계학의의。결론신암조직중NHERF3단백급mRNA여암방조직비교균정현이상저표체，표명NHERF3기인표체재전록수평수도명현억제，제시여신암NHERF3단백결핍상관적NHERF3공능결실( loss of function)가능재신암발생、발전중기중요작용。
Objective To analysis the difference of the expression level of NHERF3 between renal cell carcinoma and its adjacent tissues. Methods To detect the expression of NHERF3 protein by immunohistochemistry and Western blotting, mRNA by RT-PCR in renal cell carcinoma and their corresponding adjacent tissues. Results NHERF3 protein and mRNA level is significantly decreased in renal cell carcinoma when compared with that in adjacent tissues. Conclusion NHERF3 gene expression was significantly inhibited at the transcriptional level.