CAJ | 학술논문

目的：利用新型有机硅材料聚二甲基硅氧烷( polydimethylsiloxane,PDMS)构建细胞组织微培养装置,建立肿瘤迁移浸润的动态分析方法,对迁移的细胞进行形态学和生物学检测。方法设计并用PDMS定制具有独立分割功能的细胞微培养装置。在人脑胶质瘤U87MG细胞中检测时间依赖的迁移行为和细胞迁移信号转化生长因子-β( transforming growth factor-beta, TGF-β)驱动的荧光素酶活性。检测肿瘤细胞在缺氧环境下迁移行为的改变,并与经典的TransWell细胞迁移检测结果进行了对比。结果应用定制PDMS培养装置能够动态观测细胞迁移浸润,通过对迁移细胞群的分离回收的细胞和相关信号通路的快速定量分析能够更加全面准确地反映细胞迁移的动态表型改变和参与机制。结论应用PDMS定制细胞微培养装置进行细胞动态描述和分析,对研究肿瘤细胞迁移过程及其机制是一个可行且极具潜力的新方法。本微培养装置的特色在于在肿瘤细胞迁移过程中同时完成对形态学和分子生物学的分析并进行相关性研究。
목적：이용신형유궤규재료취이갑기규양완( polydimethylsiloxane,PDMS)구건세포조직미배양장치,건립종류천이침윤적동태분석방법,대천이적세포진행형태학화생물학검측。방법설계병용PDMS정제구유독립분할공능적세포미배양장치。재인뇌효질류U87MG세포중검측시간의뢰적천이행위화세포천이신호전화생장인자-β( transforming growth factor-beta, TGF-β)구동적형광소매활성。검측종류세포재결양배경하천이행위적개변,병여경전적TransWell세포천이검측결과진행료대비。결과응용정제PDMS배양장치능구동태관측세포천이침윤,통과대천이세포군적분리회수적세포화상관신호통로적쾌속정량분석능구경가전면준학지반영세포천이적동태표형개변화삼여궤제。결론응용PDMS정제세포미배양장치진행세포동태묘술화분석,대연구종류세포천이과정급기궤제시일개가행차겁구잠력적신방법。본미배양장치적특색재우재종류세포천이과정중동시완성대형태학화분자생물학적분석병진행상관성연구。
Objective To develop assays for the dynamic analyses for the migratory invasion of tumor through biocompatible polydimethylsiloxane( PDMS) materials to produce tissue culture apparatus, thus to allow simultaneous morphological and biochemical measurements. Methods An adaptive PDMS mini-culture apparatus with a separate blade was designed and manufactured. U87MG glioblastoma cells were analyzed for time-dependent migration in correlation with the transforming growth factor-beta ( TGF-β) luciferase reporter activities. The cell behavior in response to hypoxia treatment was investigated and compared with the results from classic TransWell assays. Results The dynamic monitoring and determination of cell invasiveness and migration could be conveniently achieved with flexibility using the customized PDMS apparatus. By separation and collection of the subpopulations of high-mobility cells, the involved signal transduction pathways could be detected with ease and precision. Conclusion The dynamic characterization and analyses using PDMS-based customized cell culture devices can be an attractive approach to investigate the process and underlining mechanisms of cancer cell migration. The exemplary apparatus demonstrated in this study showed promising potentials for the coordinated investigation from both morphological and biochemical measurements during the tumor cell migration process.