CAJ | 학술논문

目的探讨微小RNA (miR)-487b对胶质瘤细胞增殖和凋亡的影响.方法通过实时荧光定量PCR(qRT-PCR)检测6例非肿瘤脑组织、6例胶质瘤组织中miR-487b的表达;体外培养人胶质瘤细胞LN229将其分为两组:对照组(转染无义序列作为对照),实验组(转染人工合成miR-487b模拟体),qRT-PCR检测转染后细胞中miR-487b的表达水平;采用MTT检测LN229细胞增殖情况;流式细胞术检测LN229细胞周期及凋亡;结果 miR-487b的表达量在胶质瘤组织中(2.67±0.90)×10-2较非肿瘤脑组织(1.23±0.22)含量低(P＜0.05),miR-487b模拟体上调了LN229细胞中miR-487b的表达水平(与对照组比较P＜0.05),MTT结果显示miR-487b能明显抑制LN229细胞的增殖(与对照组比较P ＜0.05);流式细胞术检测细胞周期发现miR-487b可将LN229细胞阻滞于G0/G1期(与对照组比较P＜0.05);流式细胞术检测细胞凋亡发现miR-487b组细胞凋亡比例较对照组明显增加(P＜0.05).结论 miR-487b可有效抑制胶质瘤细胞增殖并促进其凋亡,可成为胶质瘤诊断和治疗的候选靶点.
목적 탐토미소RNA (miR)-487b대효질류세포증식화조망적영향.방법 통과실시형광정량PCR(qRT-PCR)검측6례비종류뇌조직、6례효질류조직중miR-487b적표체;체외배양인효질류세포LN229장기분위량조:대조조(전염무의서렬작위대조),실험조(전염인공합성miR-487b모의체),qRT-PCR검측전염후세포중miR-487b적표체수평;채용MTT검측LN229세포증식정황;류식세포술검측LN229세포주기급조망;결과 miR-487b적표체량재효질류조직중(2.67±0.90)×10-2교비종류뇌조직(1.23±0.22)함량저(P＜0.05),miR-487b모의체상조료LN229세포중miR-487b적표체수평(여대조조비교P＜0.05),MTT결과현시miR-487b능명현억제LN229세포적증식(여대조조비교P ＜0.05);류식세포술검측세포주기발현miR-487b가장LN229세포조체우G0/G1기(여대조조비교P＜0.05);류식세포술검측세포조망발현miR-487b조세포조망비례교대조조명현증가(P＜0.05).결론 miR-487b가유효억제효질류세포증식병촉진기조망,가성위효질류진단화치료적후선파점.
Objective To investigate the effect of microRNA (miR)-487b on the proliferation and apoptosis of glioma cells by observing the changes of cell proliferation,cell cycle and apoptosis.Methods The expression of miR-487b in 6 samples of glioma tissues and 6 samples of non-tumor control brain tissues was detected by using real-time PCR (qRT-PCR).After artificially synthesized miR-487b mimics was transiently transfected into LN229 glioma cells,the expression of miRNA-487b was tested by qRT-PCR.MTT assay was applied to detect the cell proliferation.The cell cycle and apoptosis were measured by flow cytometry.Results MiR-487b was down-regulated in glioma samples(2.67 ±0.90) × 10-2 compared with non-tumor samples (1.23 ± 0.22).The transient transfection of miR-487b mimics into LN229 glioma cells significantly increased the expression of miR-487b (compared with normal control,P ＜ 0.05).The result of MTT assay showed that miR-487b might inhibit LN229 cells proliferation significantly (compared to normal control P ＜ 0.05).The cell cycle analysis detected by flow cytometry assay showed that miR-487b raised the cell proportion in G0/G1 phase (compared to normal control,P ＜0.05).The apoptosis rate detected by flow cytometry assay showed that miR-487b might promote the cell apoptosis (compared with normal control,P ＜ 0.05).Conclusion MiR-487b might decrease glioma cells proliferation,block cell cycle,and promote cell apoptosis,which indicated that miR-487b was a new target for the diagnosis and treatment of glioma.