中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2015年
3期
304-308
,共5页
韩春雷%孟凡刚%刘阳%王开亮%赵学敏%张鑫%张建国
韓春雷%孟凡剛%劉暘%王開亮%趙學敏%張鑫%張建國
한춘뢰%맹범강%류양%왕개량%조학민%장흠%장건국
癫痫%大鼠%基因表达谱%长链非编码RNA
癲癇%大鼠%基因錶達譜%長鏈非編碼RNA
전간%대서%기인표체보%장련비편마RNA
Epilepsy%Rats%Gene expression profiling%RNA,long noncoding
目的 利用基因芯片筛选癫痫大鼠海马组织中差异表达的长链非编码RNA(lncRNAs)和mRNAs,分析lncRNAs在癫痫发病中的可能作用.方法 建立癫痫大鼠模型,提取海马RNA,利用Rat Gene 2.0 ST微阵列芯片分别检测5例癫痫及5例健康大鼠海马组织的lncRNAs和mRNAs表达,对差异表达的mRNAs进行GO、Pathway分析,构建lncRNAs和mRNAs的共表达网络,预测lncRNAs的可能功能.结果 按癫痫组与对照组的基因转录产物表达倍数大于1.2并且统计量P ＜0.05为标准筛选,得到差异lncRNAs 198个(上调92个,下调106个),差异表达mRNAs 1 804个(上调983个,下调821个).差异表达的mRNAs涉及离子转运、缺氧反应、细胞粘附、炎性反应等功能.Pathway分析显示,差异表达mRNAs在MAPK信号通路、局部粘附、p53信号通路、凋亡等方面功能的基因有显著地变化.生物信息学分析可初步预测差异lncRNAs可能的生物学功能及作用靶点.结论 本研究发现了癫痫大鼠海马组织中差异表达的lncRNAs和mRNAs,lncRNAs可能通过调控mRNAs的表达参与癫痫的发病或发展.
目的 利用基因芯片篩選癲癇大鼠海馬組織中差異錶達的長鏈非編碼RNA(lncRNAs)和mRNAs,分析lncRNAs在癲癇髮病中的可能作用.方法 建立癲癇大鼠模型,提取海馬RNA,利用Rat Gene 2.0 ST微陣列芯片分彆檢測5例癲癇及5例健康大鼠海馬組織的lncRNAs和mRNAs錶達,對差異錶達的mRNAs進行GO、Pathway分析,構建lncRNAs和mRNAs的共錶達網絡,預測lncRNAs的可能功能.結果 按癲癇組與對照組的基因轉錄產物錶達倍數大于1.2併且統計量P ＜0.05為標準篩選,得到差異lncRNAs 198箇(上調92箇,下調106箇),差異錶達mRNAs 1 804箇(上調983箇,下調821箇).差異錶達的mRNAs涉及離子轉運、缺氧反應、細胞粘附、炎性反應等功能.Pathway分析顯示,差異錶達mRNAs在MAPK信號通路、跼部粘附、p53信號通路、凋亡等方麵功能的基因有顯著地變化.生物信息學分析可初步預測差異lncRNAs可能的生物學功能及作用靶點.結論 本研究髮現瞭癲癇大鼠海馬組織中差異錶達的lncRNAs和mRNAs,lncRNAs可能通過調控mRNAs的錶達參與癲癇的髮病或髮展.
목적 이용기인심편사선전간대서해마조직중차이표체적장련비편마RNA(lncRNAs)화mRNAs,분석lncRNAs재전간발병중적가능작용.방법 건립전간대서모형,제취해마RNA,이용Rat Gene 2.0 ST미진렬심편분별검측5례전간급5례건강대서해마조직적lncRNAs화mRNAs표체,대차이표체적mRNAs진행GO、Pathway분석,구건lncRNAs화mRNAs적공표체망락,예측lncRNAs적가능공능.결과 안전간조여대조조적기인전록산물표체배수대우1.2병차통계량P ＜0.05위표준사선,득도차이lncRNAs 198개(상조92개,하조106개),차이표체mRNAs 1 804개(상조983개,하조821개).차이표체적mRNAs섭급리자전운、결양반응、세포점부、염성반응등공능.Pathway분석현시,차이표체mRNAs재MAPK신호통로、국부점부、p53신호통로、조망등방면공능적기인유현저지변화.생물신식학분석가초보예측차이lncRNAs가능적생물학공능급작용파점.결론 본연구발현료전간대서해마조직중차이표체적lncRNAs화mRNAs,lncRNAs가능통과조공mRNAs적표체삼여전간적발병혹발전.
Objective To detect differentially expressed lncRNAs and mRNAs in hippocampus from epileptic rats using microarray and explore the role of lncRNAs in the pathogenesis of epilepsy.Methods The lithium-pilocarpine-induced status epilepticus model was established in Sprague-Dawley rats.Total RNA samples were isolated from hippocampus of 5 epileptic rats and 5 normal rats 24 hours after the induction of status epilepticus.Rat Gene 2.0 ST microarray was used to detect deregulated lncRNAs and mRNAs in the hippocampus.GO and Pathway analysis was performed.The coding-noncoding gene co-expression network was established.Results A total of 198 deregulated lncRNAs and 1 804 deregulated mRNAs were detected in epileptic rats.GO Term enrichment in the differentially expressed mRNAs list included ion transport,response to hydrogen peroxide,cell adhesion,inflammatory response etc.Differentially expressed mRNAs might involve in MAPK signaling pathway,focal adhesion,p53 signaling pathway,apoptosis,etc.Target regulated by lncRNAs was predicted with bioinformatic prediction.Conclusions This study explored the lncRNAs and mRNAs expression in epileptic rats using microarray.Differentially expressed lncRNAs might play a role in the pathogenesis of temporal lobe epilepsy.
