CAJ | 학술논문

通过富集、驯化、分离，从清远市电子废弃物拆解区污染土壤中得到四种耐性菌株，经菌落形态、扫描电镜分析以及16S rDNA技术鉴定得出菌株HS-01、JH-02、YB-03、JY-04分别为海水芽孢八叠球菌（Sporosarcina aquimarina）、佐吕间湖生芽孢八叠球菌（Sporosarcina saromensis）、巨大芽孢杆菌（Bacillus megaterium）、甲基营养型芽孢杆菌（Bacillus methylotrophicus）。选取非矿山型东南景天（Sedum alfredii）为实验植物，在温室盆栽实验条件下，强化修复模拟的Cd、Cu、Pb污染土壤。结果显示添加细菌JH-02的东南景天地上部重金属提取量相对最高，JY-04次之，均显著高于其他各组（P﹤0.05）；重金属形态分析显示细菌JH-02与JY-04对土壤重金属的活化作用相对最好，可交换态含量明显高于其他两种细菌组与未加菌组（P﹤0.05）。实验结果表明，JH-02与JY-04这两种细菌可能通过代谢活动产生的分泌物溶解了土壤中重金属，最终促进了东南景天的提取以及根部重金属向茎叶中转移，可达到强化修复的效果，具有一定的应用前景。
통과부집、순화、분리，종청원시전자폐기물탁해구오염토양중득도사충내성균주，경균락형태、소묘전경분석이급16S rDNA기술감정득출균주HS-01、JH-02、YB-03、JY-04분별위해수아포팔첩구균（Sporosarcina aquimarina）、좌려간호생아포팔첩구균（Sporosarcina saromensis）、거대아포간균（Bacillus megaterium）、갑기영양형아포간균（Bacillus methylotrophicus）。선취비광산형동남경천（Sedum alfredii）위실험식물，재온실분재실험조건하，강화수복모의적Cd、Cu、Pb오염토양。결과현시첨가세균JH-02적동남경천지상부중금속제취량상대최고，JY-04차지，균현저고우기타각조（P﹤0.05）；중금속형태분석현시세균JH-02여JY-04대토양중금속적활화작용상대최호，가교환태함량명현고우기타량충세균조여미가균조（P﹤0.05）。실험결과표명，JH-02여JY-04저량충세균가능통과대사활동산생적분비물용해료토양중중금속，최종촉진료동남경천적제취이급근부중금속향경협중전이，가체도강화수복적효과，구유일정적응용전경。
Qingyuan is one of the biggest electronic waste(e-waste)dismantling areas in China. Over 20-year operations of individual e-waste workshops there have resulted in severe pollution of the surrounding soils by Cd, Cu and Pb. Remediation of e-waste polluted soils has drawn scientific and public attentions. In our previous study, four strains of metal tolerant bacteria were identified from the soils contaminat-ed by e-wastes in Qingyuan, using colony morphology analysis, scanning electron microscope and 16S rDNA technology. They were Sporosarcina aquimarina(HS-01), Sporosarcina saromensis(JH-02), Bacillus megaterium(YB-03)and Bacillus methylotrophicus(JY-04). Here we used these four strains and a non-contaminated ecotype of plant Sedum alfredii to test their potential for remediating soils pol-luted by Cd, Cu and Pb under greenhouse conditions. Results show that the shoots of S. alfredii accumulated the highest amount of heavy metals in the presence of JH-02 bacteria, followed by JY-04 bacteria. Bacteria JH-02 and JY-04 exhibited better performance in mobiliz-ing heavy metals, and exchangeable metal fraction was significantly higher in the JH-02 and JY-04 treatments than in other bacterial treat-ments(P﹤0.05). Our present study suggest that the bacteria JH-02 and JY-04 may be able to enhance the extraction of soil heavy metals by S. alfredii, and thus have a potential to be applied to the field.