中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2015年
3期
4-7
,共4页
吴亚文%李志红%杨佳冰%张玉玲%李莉娟%安泓霏%王玉梅%王晓亮%张和平%张学军%王磊
吳亞文%李誌紅%楊佳冰%張玉玲%李莉娟%安泓霏%王玉梅%王曉亮%張和平%張學軍%王磊
오아문%리지홍%양가빙%장옥령%리리연%안홍비%왕옥매%왕효량%장화평%장학군%왕뢰
奶牛%隐性乳房炎%病原菌%分离%鉴定%药敏试验
奶牛%隱性乳房炎%病原菌%分離%鑒定%藥敏試驗
내우%은성유방염%병원균%분리%감정%약민시험
dairy cow%sub-mastitis%bacteria%isolation%identification%drug sensitivity test
[目的]了解宁夏地区奶牛隐性乳房炎主要病原菌流行情况及主要病原菌的耐药特征。[方法]采集宁夏地区5个规模化奶牛养殖场乳样共计461份，分离培养后，应用全自动细菌鉴定仪进行鉴定并选择主要致病菌进行药敏试验。[结果]隐性乳房炎阳性检出率平均为65.3%（301/461）；分离到金黄色葡萄球菌22株、无乳链球菌5株、大肠杆菌10株。这些细菌对阿米卡星、头孢噻肟等药物敏感，对青霉素与环丙沙星耐药。[结论]宁夏地区奶牛隐性乳房炎的阳性率较高，主要病原菌对阿米卡星、头孢噻肟等药物敏感。
[目的]瞭解寧夏地區奶牛隱性乳房炎主要病原菌流行情況及主要病原菌的耐藥特徵。[方法]採集寧夏地區5箇規模化奶牛養殖場乳樣共計461份，分離培養後，應用全自動細菌鑒定儀進行鑒定併選擇主要緻病菌進行藥敏試驗。[結果]隱性乳房炎暘性檢齣率平均為65.3%（301/461）；分離到金黃色葡萄毬菌22株、無乳鏈毬菌5株、大腸桿菌10株。這些細菌對阿米卡星、頭孢噻肟等藥物敏感，對青黴素與環丙沙星耐藥。[結論]寧夏地區奶牛隱性乳房炎的暘性率較高，主要病原菌對阿米卡星、頭孢噻肟等藥物敏感。
[목적]료해저하지구내우은성유방염주요병원균류행정황급주요병원균적내약특정。[방법]채집저하지구5개규모화내우양식장유양공계461빈，분리배양후，응용전자동세균감정의진행감정병선택주요치병균진행약민시험。[결과]은성유방염양성검출솔평균위65.3%（301/461）；분리도금황색포도구균22주、무유련구균5주、대장간균10주。저사세균대아미잡성、두포새우등약물민감，대청매소여배병사성내약。[결론]저하지구내우은성유방염적양성솔교고，주요병원균대아미잡성、두포새우등약물민감。
[Objective] To clarify the species and distribution of cow sub-mastitis pathogens in Ningxia Hui Autonomous Region and identify the antimicrobial resistance of the main pathogens. [Method] 461 milk samples were collected from dairy cows with subclinical mastitis in five dairy farms for isolation and identification of pathogenic bacteria. Identification and drug sensitivity tests of the prominent pathogens were conducted by means of automatic bacteria identification equipment. [Result] The results indicated that the positive rate in the sub-mastitis cows was 65.3%(301/461). Three pathogenic bacteria were isolated,including 22 Staphylococcus aureus strains,5 Streptococcus agalactiae strains and 10 Escherichia coli strains. These bacteria were resistant to penicillin and ciprofloxacin,but all showed susceptibility to cefotaxime and amikacin. [Conclusion] Pathogens isolated and identified in this experiment were susceptible to cefotaxime and amikacin.
