CAJ | 학술논문

目的：检测纤维蛋白胶对人脂肪源干细胞增殖的影响。方法：体外培养人脂肪源干细胞，取第三代细胞接种到96孔板，以不同浓度（纤维蛋白与凝血酶比例：4∶1、2∶1、8∶3、5∶2）的纤维蛋白胶浸润提取液培养，用MTT法检测脂肪源干细胞在不同浓度纤维蛋白胶上的增殖情况。结果：不同浓度纤维蛋白胶制备的浸泡提取液所培养的人脂肪源干细胞的生长曲线无明显差异。结论：不同浓度纤维蛋白胶对人脂肪源干细胞的增殖无明显影响，纤维蛋白胶有望成为人脂肪源干细胞生长的支架材料。
목적：검측섬유단백효대인지방원간세포증식적영향。방법：체외배양인지방원간세포，취제삼대세포접충도96공판，이불동농도（섬유단백여응혈매비례：4∶1、2∶1、8∶3、5∶2）적섬유단백효침윤제취액배양，용MTT법검측지방원간세포재불동농도섬유단백효상적증식정황。결과：불동농도섬유단백효제비적침포제취액소배양적인지방원간세포적생장곡선무명현차이。결론：불동농도섬유단백효대인지방원간세포적증식무명현영향，섬유단백효유망성위인지방원간세포생장적지가재료。
Objective To examine whether the fibrin glue affects human adipose tissue-derived stem cells proliferation. Methods Human adipose tissue-derived stem cells were cultured in vitro to passage three and seeded on 96-well plate,cultured with fibrin glue in different concentration (fibrinogen/thrombin:4:1,2:1,8:3,5:2),then examine the effects on human adipose tissue-derived stem cells proliferation in different concentration by MTT assay. Results Fibrin glue in different concentration were no difference among cell growth curves of human adipose tissue-derived stem cells proliferation. Conclusion Fibrin glue in different concentration does not affect human adipose tissue-derived stem cells proliferation and may be a suitable materials as a scaffold composite with human adipose tissue-derived stem cells.