CAJ | 학술논문

目的：探讨内吗啡肽在视网膜的分布，内吗啡肽和μ阿片受体在视网膜的共存关系。方法成年雄性 SD 大鼠12只，分为2组，一组为正常对照组（n ＝6），另一组建立高眼压模型（高眼压模型组，n ＝6）。运用免疫组织化学方法观察内吗啡肽1（EM-1）、内吗啡肽2（EM-2）和μ阿片受体在视网膜中的分布和共存，并利用高眼压模型观察高眼压对 EM-2在视网膜表达的影响。结果视网膜无 EM-1分布；EM-2主要分布于视网膜节细胞层；EM-2与 NeuN、μ阿片受体在视网膜节细胞层共存；高眼压模型大鼠视网膜节细胞层内单位面积表达 EM-2的细胞数明显少于正常大鼠（0.8±0.1个／mm3 vs.4.6±0.8个／mm3），差异有统计学意义（t ＝3.88，P<0.05）。结论EM-2分布在视网膜节细胞层，与μ阿片受体共存，在高眼压状态下，EM-2的表达减少。
목적：탐토내마배태재시망막적분포，내마배태화μ아편수체재시망막적공존관계。방법성년웅성 SD 대서12지，분위2조，일조위정상대조조（n ＝6），령일조건립고안압모형（고안압모형조，n ＝6）。운용면역조직화학방법관찰내마배태1（EM-1）、내마배태2（EM-2）화μ아편수체재시망막중적분포화공존，병이용고안압모형관찰고안압대 EM-2재시망막표체적영향。결과시망막무 EM-1분포；EM-2주요분포우시망막절세포층；EM-2여 NeuN、μ아편수체재시망막절세포층공존；고안압모형대서시망막절세포층내단위면적표체 EM-2적세포수명현소우정상대서（0.8±0.1개／mm3 vs.4.6±0.8개／mm3），차이유통계학의의（t ＝3.88，P<0.05）。결론EM-2분포재시망막절세포층，여μ아편수체공존，재고안압상태하，EM-2적표체감소。
Objective To investigate the distribution of endomorphins (EM)in the retina,and as-sess the coexistence of EMand μ-opioid receptor in the retina.Methods Twelve Sprague Dawley (SD)rats were divided into the control (n =6)and intraocular hypertension model groups (n =6).Immunohistochemis-try was used to detect the expression and coexistence of EM-1,EM-2 and μ-opioid receptor in the rat retina, and evaluate the influence of intraocular hypertension upon the expression of EM-2.Results EM-1 was not expressed in the retina.EM-2 was mainly distributed in the retinal ganglion cell layer.EM-2,NeuN and μ-opioid receptor coexisted in the retinal ganglion cell layer.The density of cells expressing EM-2 in the intraocu-lar hypertension model group was (0.8 ±0.1) /mm3 ,significantly lower compared with (4.6 ±0.8) /mm3 in the control group (t =3.88,P < 0.05).Conclusions EM-2 was expressed in the retinal ganglion cell layer and coexisted with μ-opioid receptor.The expression of EM-2 was decreased under intraocular hyperten-sion.