江苏科技信息
江囌科技信息
강소과기신식
JIANGSU SCIENCE & TECHNOLOGY INFORMATION
2015年
7期
41-42
,共2页
髙莉莉%徐苏娟%张继明%王建杰
髙莉莉%徐囌娟%張繼明%王建傑
고리리%서소연%장계명%왕건걸
结核分枝杆菌%HspX%真核表达
結覈分枝桿菌%HspX%真覈錶達
결핵분지간균%HspX%진핵표체
mycobacterium tuberculosis%HspX%eukaryotic expression
为构建结核分枝杆菌HspX基因与增强型绿色荧光蛋白（EGFP）融合基因的真核表达载体，文章采用PCR法扩增HspX基因，定向插入到质粒pEGFP-N1的多克隆位点。结果显示，融合基因真核表达载体，经双酶切鉴定、基因测序鉴定克隆片段大小和序列正确。文章成功构建了pEGFP-N1-HspX融合基因真核表达载体。
為構建結覈分枝桿菌HspX基因與增彊型綠色熒光蛋白（EGFP）融閤基因的真覈錶達載體，文章採用PCR法擴增HspX基因，定嚮插入到質粒pEGFP-N1的多剋隆位點。結果顯示，融閤基因真覈錶達載體，經雙酶切鑒定、基因測序鑒定剋隆片段大小和序列正確。文章成功構建瞭pEGFP-N1-HspX融閤基因真覈錶達載體。
위구건결핵분지간균HspX기인여증강형록색형광단백（EGFP）융합기인적진핵표체재체，문장채용PCR법확증HspX기인，정향삽입도질립pEGFP-N1적다극륭위점。결과현시，융합기인진핵표체재체，경쌍매절감정、기인측서감정극륭편단대소화서렬정학。문장성공구건료pEGFP-N1-HspX융합기인진핵표체재체。
In order to construct a recombinant plasmid carrying enhanced green fluorescent protein (EGFP)and Mycobacterium tuberculosis HspX gene,HspX gene was amplified by PCR and inserted into a multiple cloning site of eukaryotic expression vector pEGFP-N1. The results shows HspX eukaryotic expression vector labeled with FLAG tag was successfully constructed as demonstrated by double enzyme digestion and DNA sequencing. The conclusion is that the eukaryotic expression plasmid pEGFP-N1-HspX has been constructed successfully.
