CAJ | 학술논문

目的：开发一种新型阿霉素脂质体制剂。方法采用膜分散法制备阿霉素脂质体；采用噻唑蓝比色法( MTT法)考察阿霉素和阿霉素脂质体对5种人类肿瘤细胞株增生的抑制作用；以荷S180小鼠为模型，考察阿霉素和阿霉素脂质体的抗肿瘤活性。结果膜分散法适用于制备阿霉素脂质体，其平均包封率高于95%；阿霉素制备成脂质体仍具备抑制肿瘤细胞株增生活性，并呈现时间依赖关系；动物实验表明，阿霉素脂质体的抗肿瘤活性比阿霉素强，毒性比阿霉素低。结论此法制备的阿霉素脂质体包封率高，简便易行，重现性好，并且显示了较好的抗肿瘤活性。
목적：개발일충신형아매소지질체제제。방법채용막분산법제비아매소지질체；채용새서람비색법( MTT법)고찰아매소화아매소지질체대5충인류종류세포주증생적억제작용；이하S180소서위모형，고찰아매소화아매소지질체적항종류활성。결과막분산법괄용우제비아매소지질체，기평균포봉솔고우95%；아매소제비성지질체잉구비억제종류세포주증생활성，병정현시간의뢰관계；동물실험표명，아매소지질체적항종류활성비아매소강，독성비아매소저。결론차법제비적아매소지질체포봉솔고，간편역행，중현성호，병차현시료교호적항종류활성。
Objective To develop a novel preparation of liposomal doxorubicin. Methods The liposome was prepared by dispersion film assay, loaded doxorubicin by ammonium sulfate gradient method, the anti-proliferation activities of doxorubicin liposome and doxorubicin against cancer cells evaluated by MTT assay and the antitumor activities of doxorubicin liposome and doxorubicin on S180 mouse model were measured. Results The doxorubicin liposome encapsulation efficiency was more than 96. 3% at different time points. Doxorubicin liposome effectively inhibited the proliferation of carcinoma cells. The in vivo anti-tumor activity and toxicity of doxorubicin liposome were significantly higher and lower than that of doxorubicin, respectively. Conclusion The method is practicable to prepare doxorubicin liposome having good antitumor potency in vivo.