CAJ | 학술논문

目的：观察胸腺基质淋巴细胞生成素（TSLP）及 C D4＋C D25＋Foxp3＋调节性 T 细胞（Tregs）在单纯慢性鼻窦炎中的表达，探讨二者在慢性鼻窦炎发生发展过程中可能的作用机制。方法收集25例慢性鼻窦炎患者的鼻窦黏膜为实验组，28例鼻中隔偏曲患者的下鼻甲黏膜为对照组，采用 Q-RT-PCR 和免疫组化技术检测各组鼻腔黏膜中调节性 T 细胞、Foxp3 mRNA 和 TSLP mRNA 的表达，并分析 TSLP 与调节性 T 细胞、Foxp3 mRNA 的相关关系。结果①TSLP mRNA、Foxp3 mRNA、调节性 T 细胞在慢性鼻窦炎中的表达明显高于对照组（P ＜0．05），TSLP 与Foxp3 mRNA 的表达在慢性鼻窦炎中呈正相关（r ＝0．9773，P ＜0．0001）；②根据免疫组化结果计数 Tregs 数量， TSLP 与调节性 T 细胞在慢性鼻窦炎中的表达也呈正相关关系（r ＝0．8646，P ＜0．0001）。结论TSLP 可通过诱导调节性 T 细胞的发育和增殖，维持免疫自稳，参与慢性鼻窦炎的发生发展过程，其具体的机制有待进一步研究。
목적：관찰흉선기질림파세포생성소（TSLP）급 C D4＋C D25＋Foxp3＋조절성 T 세포（Tregs）재단순만성비두염중적표체，탐토이자재만성비두염발생발전과정중가능적작용궤제。방법수집25례만성비두염환자적비두점막위실험조，28례비중격편곡환자적하비갑점막위대조조，채용 Q-RT-PCR 화면역조화기술검측각조비강점막중조절성 T 세포、Foxp3 mRNA 화 TSLP mRNA 적표체，병분석 TSLP 여조절성 T 세포、Foxp3 mRNA 적상관관계。결과①TSLP mRNA、Foxp3 mRNA、조절성 T 세포재만성비두염중적표체명현고우대조조（P ＜0．05），TSLP 여Foxp3 mRNA 적표체재만성비두염중정정상관（r ＝0．9773，P ＜0．0001）；②근거면역조화결과계수 Tregs 수량， TSLP 여조절성 T 세포재만성비두염중적표체야정정상관관계（r ＝0．8646，P ＜0．0001）。결론TSLP 가통과유도조절성 T 세포적발육화증식，유지면역자은，삼여만성비두염적발생발전과정，기구체적궤제유대진일보연구。
Objective To determine the expression of thymic stromal lymphopoietin(TSLP)and regulatory T cells (C D4 +C D25 + Foxp3 + Treg)in human chronic rhinosinusitis (CRS ) and to analyze their pathogenesis in CRS. Methods The sinus mucosa from 25 patients with CRS was studied.Inferior nasal turbinate from 28 patients with nasal septum deviation served as the control.Their histological characteristics were analyzed by HE stain.The expressions of TSLP and Foxp3 mRNA were detected by Q-RT-PCR.Immunohistochemistry was used to detect C D4 +C D25 +Foxp3 +Treg cells.The correlation of TSLP with the number of Tregs and the correlation of TSLP with Foxp3 mRNA were evaluated.Results The expressions of TSLP mRNA,Foxp3 mRNA and Treg cells were significant higher in CRS compared with the control group(P <0.05).There was positive correlation between TSLP and Foxp3 mRNA(r =0.977 3, P <0.000 1).At the same time,there was positive correlation between TSLP and the number of Tregs(r =0.864 6,P <0.0001).Conclusion TSLP could play an important role in CRS by inducing Treg cells.