农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2015年
4期
672-678
,共7页
徐红霞%李宵慧%孙媛媛%施小清%吴吉春
徐紅霞%李宵慧%孫媛媛%施小清%吳吉春
서홍하%리소혜%손원원%시소청%오길춘
荧蒽%草螺菌属细菌%生物降解%固定化%响应曲面法
熒蒽%草螺菌屬細菌%生物降解%固定化%響應麯麵法
형은%초라균속세균%생물강해%고정화%향응곡면법
fluoranthene%Herbaspirillum chlorophenolicum strain FA1%biodegradation%immobilization%response surface methodology
对草螺菌属荧蒽降解菌Herbaspirillum chlorophenolicum strain FA1以不同材料和方法进行固定化,考察固定化后菌株对土水系统中荧蒽的降解性能。结果表明:聚乙烯醇(PVA)-硅藻土载体(硼酸法)固定的菌株对荧蒽的降解效率最高,对荧蒽的25-d降解率达97.74%;硼酸法制备的PVA-硅藻土载体和PVA-活性炭载体对荧蒽的降解效果优于相应冷冻-解冻法制得的载体;硅藻土的添加比活性炭更能够提高固定在PVA复合载体中的菌株FA1对荧蒽的降解率。在此基础上,对PVA-硅藻土载体(硼酸法)进行响应曲面法优化,采用4水平的Box-Behnken设计安排实验,对结果进行拟合和分析,建立二次回归模型并求解,获得最佳固定化条件为:菌浓度9.6%、PVA浓度11.2%、硅藻土浓度4.5%、粒径4 mm。据此优化后,PVA-硅藻土载体(硼酸法)对荧蒽的20-d降解率即达99.46%。
對草螺菌屬熒蒽降解菌Herbaspirillum chlorophenolicum strain FA1以不同材料和方法進行固定化,攷察固定化後菌株對土水繫統中熒蒽的降解性能。結果錶明:聚乙烯醇(PVA)-硅藻土載體(硼痠法)固定的菌株對熒蒽的降解效率最高,對熒蒽的25-d降解率達97.74%;硼痠法製備的PVA-硅藻土載體和PVA-活性炭載體對熒蒽的降解效果優于相應冷凍-解凍法製得的載體;硅藻土的添加比活性炭更能夠提高固定在PVA複閤載體中的菌株FA1對熒蒽的降解率。在此基礎上,對PVA-硅藻土載體(硼痠法)進行響應麯麵法優化,採用4水平的Box-Behnken設計安排實驗,對結果進行擬閤和分析,建立二次迴歸模型併求解,穫得最佳固定化條件為:菌濃度9.6%、PVA濃度11.2%、硅藻土濃度4.5%、粒徑4 mm。據此優化後,PVA-硅藻土載體(硼痠法)對熒蒽的20-d降解率即達99.46%。
대초라균속형은강해균Herbaspirillum chlorophenolicum strain FA1이불동재료화방법진행고정화,고찰고정화후균주대토수계통중형은적강해성능。결과표명:취을희순(PVA)-규조토재체(붕산법)고정적균주대형은적강해효솔최고,대형은적25-d강해솔체97.74%;붕산법제비적PVA-규조토재체화PVA-활성탄재체대형은적강해효과우우상응냉동-해동법제득적재체;규조토적첨가비활성탄경능구제고고정재PVA복합재체중적균주FA1대형은적강해솔。재차기출상,대PVA-규조토재체(붕산법)진행향응곡면법우화,채용4수평적Box-Behnken설계안배실험,대결과진행의합화분석,건립이차회귀모형병구해,획득최가고정화조건위:균농도9.6%、PVA농도11.2%、규조토농도4.5%、립경4 mm。거차우화후,PVA-규조토재체(붕산법)대형은적20-d강해솔즉체99.46%。
Microbial remediation of polycyclic aromatic hydrocarbons(PAHs), a group of persistent toxic contaminants that are widespread in terrestrial and aquatic environments, has received increasing interests. A fluoranthene-degrading strain FA1 previously isolated from acti-vated sludge has showed capability to use fluoranthene as sole carbon and energy sources and to degrade fluoranthene in the presence of in-digenous flora. In this study, immobilization of fluoranthene-degrading strain FA1 by various matrices and its effects on fluoranthene degra-dation in soil-water system were investigated. Results showed that the strain FA1 immobilized on polyvinyl alcohol(PVA)-diatomite matrix prepared by chemical method(boric acid)exhibited the highest degradation of fluoranthene, with a 97.74% removal of fluoranthene from soil-water system after 25 d. PVA-diatomite and PVA-activated carbon matrices obtained by chemical method showed better effectiveness than those obtained by freezing and thawing method. The optimal immobilization conditions of PVA- diatomite matrix(chemical method) were 9.6%of cell density, 11.2%of PVA, 4.5%of diatomite and 4.0 mm of bead size. Under these conditions, 99.46%of fluoranthene was removed from the soil-water system after 20 d.