CAJ | 학술논문

目的：比较采用不同药敏检测方法检测替加环素对鲍曼不动杆菌敏感性的差异。方法2013年临床分离碳青霉烯耐药鲍曼不动杆菌60株，采用微量肉汤稀释法、M TS测试条法、Vitek2仪器检测法、纸片扩散法分别检测替加环素对鲍曼不动杆菌的敏感性。结果微量肉汤稀释法替加环素敏感性为100．00％，未检测到中介和耐药菌株；M T S测试条法敏感性为81．67％，中介率为18．33％，未检测到耐药菌株；Vitek2仪器法敏感性为50．00％，中介率为43．33％，耐药率为6．67％；纸片扩散法敏感性为45．00％，中介率为45．00％，耐药率为10．00％。结论替加环素对碳青霉烯耐药鲍曼不动杆菌具有较好的抗菌活性，但检测方法的局限性对结果的报告影响较大，以微量肉汤法为标准，M TS测试条法的符合率最高，Vitek2仪器法和纸片扩散法的敏感性显著降低。
목적：비교채용불동약민검측방법검측체가배소대포만불동간균민감성적차이。방법2013년림상분리탄청매희내약포만불동간균60주，채용미량육탕희석법、M TS측시조법、Vitek2의기검측법、지편확산법분별검측체가배소대포만불동간균적민감성。결과미량육탕희석법체가배소민감성위100．00％，미검측도중개화내약균주；M T S측시조법민감성위81．67％，중개솔위18．33％，미검측도내약균주；Vitek2의기법민감성위50．00％，중개솔위43．33％，내약솔위6．67％；지편확산법민감성위45．00％，중개솔위45．00％，내약솔위10．00％。결론체가배소대탄청매희내약포만불동간균구유교호적항균활성，단검측방법적국한성대결과적보고영향교대，이미량육탕법위표준，M TS측시조법적부합솔최고，Vitek2의기법화지편확산법적민감성현저강저。
Objective To compare the difference by adopting different susceptibility testing methods to detect the susceptibility of Acinetobacter baumannii to tigecycline .Methods 60 strains of carbapenem‐resistant Acinetobacter baumannii isolated during 2013 were performed the susceptibility test to tigecycline by the broth dilution method ,MIC test strip ,VITEK 2 compact system and disk diffusion method respectively .Results The susceptibility rates of 60 strains of Acinetobacter baumannii to tigecycline by the broth dilution method ,MIC test strip ,MIC test strip were 100 .00% ,81 .67% ,50 .00% and 45 .00% respectively ,the intermedi‐ate rates were 0 .00% ,18 .33% ,43 .33% and 45 .00% ,the resistant rates were 0 .00% ,0 .00% ,6 .67% and 10 .00% respectively . Conclusion Tigecycline displays better antibacterial activity to carbapenem‐resistant Acinetobacter baumannii ,but the limitation of detection method has larger influence on the result report .With the broth dilution method as the standard ,the MIC test strip has the highest coincidence rate ,while the sensitivity of MIC test strip is significantly decreased .