CAJ | 학술논문

目的：探讨及评价乙肝5项ELISA使用水浴箱与快速孵育器实验的结果比对的意义。方法随机选取本院2012年6月至2013年6月收治的乙肝患者92例，随机选取健康体检者184例作为对照，采用水浴箱和快速孵育器用ELISA同时检测标本的乙肝5项。结果2组结果阳性符合率较高。水浴箱的标本OD值的平均值会比较高。但是快速孵育器的标本S／CO值平均值较高，与其CO值比水浴箱的标本高有关系。结论日常工作中可引进快速孵育器提高工作效率，但是“弱反应性”结果的标本，需要使用水浴箱复查，并做好记录。快速孵育器最好只用于 HBsAg的反应，因其他四项的反应流程时间比较短，并且有部分结果不符合。
목적：탐토급평개을간5항ELISA사용수욕상여쾌속부육기실험적결과비대적의의。방법수궤선취본원2012년6월지2013년6월수치적을간환자92례，수궤선취건강체검자184례작위대조，채용수욕상화쾌속부육기용ELISA동시검측표본적을간5항。결과2조결과양성부합솔교고。수욕상적표본OD치적평균치회비교고。단시쾌속부육기적표본S／CO치평균치교고，여기CO치비수욕상적표본고유관계。결론일상공작중가인진쾌속부육기제고공작효솔，단시“약반응성”결과적표본，수요사용수욕상복사，병주호기록。쾌속부육기최호지용우 HBsAg적반응，인기타사항적반응류정시간비교단，병차유부분결과불부합。
Objective To investigate and evaluate the comparison results of five items of hepatitis B in ELISA by using water bath box and rapid incubation .Methods 92 patients with hepatitis B in our hospital from June 2012 to June 2013 were randomly se‐lected and 184 cases of healthy physical examination were randomly selected as the controls .The water bath and rapid incubation were adopted to conduct ELISA for simultaneously detecting 5 items of hepatitis B in samples .Results The results of two groups had higher positive coincidence rate .The average OD value of specimens in the water bath was higher .But the specimen S/CO value in the fast average incubation was higher ,its co value was higher than that of the water bath .Conclusion The rapid incubation can be introduced for improving the work efficiency in the daily work ,but the specimen with "weak reactive"result needs to be re‐de‐tected by using the water bath and making records .The fast incubation device is best for only use of the HBsAg reaction ,because the other four reaction process time is shorter ,and there are some non‐conformity results .