国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
7期
920-922
,共3页
丙型肝炎病毒%抗原%抗体%联合检测%血液透析
丙型肝炎病毒%抗原%抗體%聯閤檢測%血液透析
병형간염병독%항원%항체%연합검측%혈액투석
hepatitis C%antigen%antibody%joint detection%hemodialysis
目的:探讨开展抗原抗体联合检测在提升血液透析治疗患者丙肝感染检出率中的应用价值。方法收集100例血液透析患者的临床资料,分别采用以下方法测定相关参数:ELISA法检测 HCV核心抗原、化学发光法检测抗 HCV 抗体、RT‐PCR法检测HCV‐RNA ,统计各检测项目在同一标本中的阳性表达情况,比较单测抗原、抗体与联合检测3个检测方案对于RNA阳性标本的检出率;同时对抗原阳性标本的抗原水平与其RN A拷贝数进行相关性分析并比较抗体阳性标本中其RN A阳性与阴性组组间抗体水平的区别。结果单测抗原或抗体均会造成血透患者丙肝感染的漏检,对于RN A阳性标本的检测中抗原抗体联合检测的检出率最高(P<0.05),抗原阳性标本的抗原水平与其对应标本的RNA拷贝数呈正相关关系(r=0.85,P<0.05),而抗体阳性标本中其RNA阳性与阴性组的组间抗体水平的差异不具有统计学意义(P>0.05)。结论 HCV抗原抗体联合检测能有效提高血液透析治疗患者丙肝感染的检出率,同时 HCV核心抗原检测也可作为抗体阳性患者 HCV再次感染的监测指标之一。
目的:探討開展抗原抗體聯閤檢測在提升血液透析治療患者丙肝感染檢齣率中的應用價值。方法收集100例血液透析患者的臨床資料,分彆採用以下方法測定相關參數:ELISA法檢測 HCV覈心抗原、化學髮光法檢測抗 HCV 抗體、RT‐PCR法檢測HCV‐RNA ,統計各檢測項目在同一標本中的暘性錶達情況,比較單測抗原、抗體與聯閤檢測3箇檢測方案對于RNA暘性標本的檢齣率;同時對抗原暘性標本的抗原水平與其RN A拷貝數進行相關性分析併比較抗體暘性標本中其RN A暘性與陰性組組間抗體水平的區彆。結果單測抗原或抗體均會造成血透患者丙肝感染的漏檢,對于RN A暘性標本的檢測中抗原抗體聯閤檢測的檢齣率最高(P<0.05),抗原暘性標本的抗原水平與其對應標本的RNA拷貝數呈正相關關繫(r=0.85,P<0.05),而抗體暘性標本中其RNA暘性與陰性組的組間抗體水平的差異不具有統計學意義(P>0.05)。結論 HCV抗原抗體聯閤檢測能有效提高血液透析治療患者丙肝感染的檢齣率,同時 HCV覈心抗原檢測也可作為抗體暘性患者 HCV再次感染的鑑測指標之一。
목적:탐토개전항원항체연합검측재제승혈액투석치료환자병간감염검출솔중적응용개치。방법수집100례혈액투석환자적림상자료,분별채용이하방법측정상관삼수:ELISA법검측 HCV핵심항원、화학발광법검측항 HCV 항체、RT‐PCR법검측HCV‐RNA ,통계각검측항목재동일표본중적양성표체정황,비교단측항원、항체여연합검측3개검측방안대우RNA양성표본적검출솔;동시대항원양성표본적항원수평여기RN A고패수진행상관성분석병비교항체양성표본중기RN A양성여음성조조간항체수평적구별。결과단측항원혹항체균회조성혈투환자병간감염적루검,대우RN A양성표본적검측중항원항체연합검측적검출솔최고(P<0.05),항원양성표본적항원수평여기대응표본적RNA고패수정정상관관계(r=0.85,P<0.05),이항체양성표본중기RNA양성여음성조적조간항체수평적차이불구유통계학의의(P>0.05)。결론 HCV항원항체연합검측능유효제고혈액투석치료환자병간감염적검출솔,동시 HCV핵심항원검측야가작위항체양성환자 HCV재차감염적감측지표지일。
Objective To invetigate the application value of antigen‐antibody joint detection in increasing the detection rate of hepatitis C infection in the patients with hemodialysis .Methods The clinical data of 100 cases of hemodialysis were collected .Hep‐atitis C virus(HCV) core antigen ,HCV antibody and HCV‐RNA were detected by adopting ELISA ,Chemiluminescence and RT‐PCR respectively .The positive expression situation of each detection item in the same specimen was performed statistics .The detec‐tion rates of RNA positive specimen were compared among the single antigen detection ,single antibody detection and joint detection schemes ;at the same time the antigen level of antigen positive specimens ant its RNA copy number were performed the correlation analysis .The antibody levels in the positive antibody specimens were compared between the RNA positive group and the RNA neg‐ative group .Results The single antigen or single antibody detection could cause the missed detection of HCV infection in hemodial‐ysis patients .For the specimens of positive RNA ,the joint detection had the highest detection rate (P<0 .05) ,the antigen level in the antigen positive specimens was positively correlated with the RNA copy number in the corresponding specimens (r=0 .85 ,P<0 .05) ,while the antibody level in the antibody positive specimens had no statistical difference between the RNA positive group and the RNA negative group(P>0 .05) .Conclusion The joint detection of HCV antigen antibody can effectively increase the detection rate of hepatitis C infection in hemodialysis patients .At the same time the HCV core antigen detection also can be used as one of monitoring indicators for the HCV re -infection in the patients with antibody positive .