CAJ | 학술논문

目的：探讨微柱凝胶免疫技术在新生儿溶血病（HDN ）筛查中的应用价值。方法采用微柱凝胶技术对本院临床疑似HDN 212例患儿血液标本做直接抗人球蛋白试验、游离试验、放散试验。结果疑似 HDN 患儿血液样本212例，确诊为HDN 50例（23．6％），其中ABO‐HDN 45例（21．2％）、Rh‐HDN 5例（2．4％）。45例ABO‐HDN的患儿中，A型23例，阳性率为36．5％（23／63）；B型22例，阳性率为28．2％（22／78）。3项试验的检出敏感性：50例HDN确诊患儿中放散试验阳性率为100％， DT A阳性率为28％，游离试验阳性率为92％。结论微柱凝胶法能快速准确检测出 HDN ，具有操作简便、标本用量少、方法学敏感性高、特异性强，可为HDN提供可靠诊断依据，适用于临床推广应用。
목적：탐토미주응효면역기술재신생인용혈병（HDN ）사사중적응용개치。방법채용미주응효기술대본원림상의사HDN 212례환인혈액표본주직접항인구단백시험、유리시험、방산시험。결과의사 HDN 환인혈액양본212례，학진위HDN 50례（23．6％），기중ABO‐HDN 45례（21．2％）、Rh‐HDN 5례（2．4％）。45례ABO‐HDN적환인중，A형23례，양성솔위36．5％（23／63）；B형22례，양성솔위28．2％（22／78）。3항시험적검출민감성：50례HDN학진환인중방산시험양성솔위100％， DT A양성솔위28％，유리시험양성솔위92％。결론미주응효법능쾌속준학검측출 HDN ，구유조작간편、표본용량소、방법학민감성고、특이성강，가위HDN제공가고진단의거，괄용우림상추엄응용。
Objective To investigate the application value of microcolumn gel technology in screening hemolytic disease of the newborns(HDN) .Methods The direct antiglobulin test(DAT) ,antibody release test and free antibody test were performed in 212 cases of suspected HDN in our hospital by using microcolumn gel assay .Results In 212 cases of suspected HDN ,50 cases(23 .6% ) were diagnosed as HDN ,including 45 cases (21 .2% ) of ABO‐HDN and 5 cases (2 .4% ) of Rh‐HDN .In 45 cases of ABO‐HDN ,23 cases (36 .5% ) were A blood type and 22 cases (28 .2% ) were B blood type .The sensitivity of antibody release test ,DAT and free antibody test was 100% ,28% and 92% respectively .Conclusion The microcolumn gel technology can detect HDN fastly and accu‐rately ,with the advantages of simple operation ,less sample consumption ,high sensitivity and specificity ,which can provide reliable basis for HDN diagnosis and is worth popularizing and applying in clinic .