中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2015年
3期
66-69
,共4页
犬弓首蛔虫%线粒体DNA%cox1基因%种系发育关系
犬弓首蛔蟲%線粒體DNA%cox1基因%種繫髮育關繫
견궁수회충%선립체DNA%cox1기인%충계발육관계
Toxocara canis%mitochondrial DNA%cox1 gene%phylogenetic relationship
本研究旨在阐明我国犬弓首蛔虫（Toxocara canis）湖南分离株线粒体细胞色素c氧化酶第I亚基（cox1）基因部分序列（pcox1）的遗传变异情况，并用其与其它弓首蛔虫的pcox1序列构建进化关系。应用PCR扩增犬弓首蛔虫虫株的pcox1，将所获得的序列应用Mafft 7.122程序进行比对，然后用PhyML 3.1程序ML法绘制种系发育树。本实验扩增所获得的pcox1序列长度一致，均为394 bp，种内变异在0~2.5%之间，种间差异为8.2%~11.6%。种系发育分析结果表明，12个犬弓首蛔虫分离株位于同一分支。由于犬弓首蛔虫pcox1序列种内相对保守，种间差异较大，故可作为种间鉴定检测研究的遗传标记，本研究结果为犬弓首蛔虫的分类、鉴定和群体遗传结构奠定了基础。
本研究旨在闡明我國犬弓首蛔蟲（Toxocara canis）湖南分離株線粒體細胞色素c氧化酶第I亞基（cox1）基因部分序列（pcox1）的遺傳變異情況，併用其與其它弓首蛔蟲的pcox1序列構建進化關繫。應用PCR擴增犬弓首蛔蟲蟲株的pcox1，將所穫得的序列應用Mafft 7.122程序進行比對，然後用PhyML 3.1程序ML法繪製種繫髮育樹。本實驗擴增所穫得的pcox1序列長度一緻，均為394 bp，種內變異在0~2.5%之間，種間差異為8.2%~11.6%。種繫髮育分析結果錶明，12箇犬弓首蛔蟲分離株位于同一分支。由于犬弓首蛔蟲pcox1序列種內相對保守，種間差異較大，故可作為種間鑒定檢測研究的遺傳標記，本研究結果為犬弓首蛔蟲的分類、鑒定和群體遺傳結構奠定瞭基礎。
본연구지재천명아국견궁수회충（Toxocara canis）호남분리주선립체세포색소c양화매제I아기（cox1）기인부분서렬（pcox1）적유전변이정황，병용기여기타궁수회충적pcox1서렬구건진화관계。응용PCR확증견궁수회충충주적pcox1，장소획득적서렬응용Mafft 7.122정서진행비대，연후용PhyML 3.1정서ML법회제충계발육수。본실험확증소획득적pcox1서렬장도일치，균위394 bp，충내변이재0~2.5%지간，충간차이위8.2%~11.6%。충계발육분석결과표명，12개견궁수회충분리주위우동일분지。유우견궁수회충pcox1서렬충내상대보수，충간차이교대，고가작위충간감정검측연구적유전표기，본연구결과위견궁수회충적분류、감정화군체유전결구전정료기출。
The objective of the study was to analyze sequence variation in the cytochrome c oxidase subunit 1(cox1) gene among Toxocara canis isolates from Hunan province and to study its phylogenetic relationship with other round-worms using the cox1 gene sequencing. The partial cox1(pcox1)were ampliifed from individual T. canis samples, and pcox1 sequences were aligned using the Mafft 7.122. Maximum likelihood(ML)trees were constructed using the software PhyML 3.1. The lengths of all pcox1 sequences were 394bp. Sequence variations in pcox1 sequences within T. canis were 0~2.5%which was signiifcantly lower than inter-species differences(8.2%~11.6%). Phylogenetic analyses showed that all T. canis isolates were clustered in the same clade. It was concluded that pcox1 sequence could be used as genetic marker for population genetic studies of T. canis,as well as genetic marker for the differentiation and identiifca-tion of different roundworms. The study provided foundation for further studies on molecular genetics and diagnosis of T. canis.
