CAJ | 학술논문

目的：探讨A型肉毒毒素（BTXA）对增生性瘢痕组织成纤维细胞中TGF-β1和cyclinD1表达的影响。方法：组织块法培养增生性瘢痕组织及正常皮肤组织的成纤维细胞，用不同浓度的BTXA作用于体外培养的人增生性瘢痕成纤维细胞，检测MTT值；利用RT-PCR分析用药前后TGF-β1和cyclinD1mRNA表达的变化及其他们的相关性。结果：BTXA在体外能明显抑制增生性瘢痕成纤维细胞的增殖及TGF-β1和cyclinD1mRNA的表达。结论：BTXA对增生性瘢痕组织中成纤维细胞的增殖有明显抑制作用，其作用机制与BTXA能抑制TGF-β1和 cyclinD1mRNA的表达有关；同时在抑制成纤维细胞的过程中，TGF-β1和cyclinD1mRNA的表达并无相关性。
목적：탐토A형육독독소（BTXA）대증생성반흔조직성섬유세포중TGF-β1화cyclinD1표체적영향。방법：조직괴법배양증생성반흔조직급정상피부조직적성섬유세포，용불동농도적BTXA작용우체외배양적인증생성반흔성섬유세포，검측MTT치；이용RT-PCR분석용약전후TGF-β1화cyclinD1mRNA표체적변화급기타문적상관성。결과：BTXA재체외능명현억제증생성반흔성섬유세포적증식급TGF-β1화cyclinD1mRNA적표체。결론：BTXA대증생성반흔조직중성섬유세포적증식유명현억제작용，기작용궤제여BTXA능억제TGF-β1화 cyclinD1mRNA적표체유관；동시재억제성섬유세포적과정중，TGF-β1화cyclinD1mRNA적표체병무상관성。
Objective To investigate the effect of botulinum toxin A (BTXA)on the expression of TGF-β1 and cyclinD1 of hypertrophic scar fibroblast. Methods After treated with different concertrations of BTXA,inhibition rate.The expression and correlation of TGF- β1 of cyclinD1of hypertrophic scar fibroblasts were detected by MTT assay and RT-PCR respectively. Results BTXA can significantly inhibit the proliferation of hypertrophic scar fibroblast,and the expression of TGF-β1 and cyclinD1 could be inhibited significantly. Conclusion The mechanism of BTXA on inhibiting fibroblast of hypertrophic scar may be related with regulation of the expression of TGF-β1 of cyclinD1,and there was no correlation between the expression of TGF-1 and cyclinD1mRNA on inhibiting fibroblast of hypertrophic scar.