CAJ | 학술논문

目的：研究大气细颗粒物对小鼠皮肤组织表皮细胞生长因子受体（epidermal growth factor receptor, EGFR） mRNA表达的影响，以探讨大气细颗粒物对皮肤老化的影响。方法：将40只BALB/c雌性小鼠随机分成生理盐水对照组及大气细颗粒物（PM2.5）染毒低、中、高剂量（分别为1.6、8.0和40.0mg/kg）组，每组10只。各剂量组均经气管滴注染毒3d。末次染毒24h后，采用实时荧光定量PCR方法分别检测各组小鼠皮肤组织EGFR mRNA的表达量。结果：与对照组相比，PM2.5染毒中、高剂量组EGFR mRNA的表达量均明显升高（P<0.05），且与剂量呈正相关。结论：中、高浓度 PM2.5可以诱导EGFR mRNA的表达，促进皮肤老化的发生。
목적：연구대기세과립물대소서피부조직표피세포생장인자수체（epidermal growth factor receptor, EGFR） mRNA표체적영향，이탐토대기세과립물대피부노화적영향。방법：장40지BALB/c자성소서수궤분성생리염수대조조급대기세과립물（PM2.5）염독저、중、고제량（분별위1.6、8.0화40.0mg/kg）조，매조10지。각제량조균경기관적주염독3d。말차염독24h후，채용실시형광정량PCR방법분별검측각조소서피부조직EGFR mRNA적표체량。결과：여대조조상비，PM2.5염독중、고제량조EGFR mRNA적표체량균명현승고（P<0.05），차여제량정정상관。결론：중、고농도 PM2.5가이유도EGFR mRNA적표체，촉진피부노화적발생。
Objective To study the influence of atmospheric fine particles on the expression of EGFR mRNA in mouse skin tissue and to explore the influence of atmospheric fine particles of skin aging. Methods 40 female BALB/c mice were randomly divided into 4 groups including 1 saline control group and 3 PM2.5 exposure groups(1.6, 8.0 and 40.0mg/kg body weight, respectively).Each exposure group received intratracheal instillation once per day for 3 consecutive days.At the 24h after the last administration,skin tissueand were collected to detect the expression of EGFR mRNA by real-time fluorescent quantitative PCR. Results Compared with control group,the expression of EGFR mRNA increased significantly in the 8.0 and 40.0mg/kg PM2.5 exposure groups (P<0.05),and positively related to dosage of PM2.5. Conclusion The 8.0 and 40.0mg/kg PM2.5 exposure can induce the expression of EGFR mRNA and promote the occurrence of skin aging.