CAJ | 학술논문

用DNAStar7.0软件对GenBank中IHNV的N基因进行序列分析，设计IHNV特异性引物并标记生物素，将探针氨基化修饰，与荧光编码微球偶联后与RT-PCR产物杂交反应，用液相芯片仪器检测荧光信号，建立了传染性造血器官坏死病毒（IHNV）的液相芯片快速检测技术。结果表明液相芯片检测体系对IHNV病毒核酸的最低检出量为100pg，且特异性高，与其他病毒无交叉反应。应用建立的液相芯片技术检测鱼类中IHNV，并与RT-PCR方法进行比较，检测结果基本一致，但该方法比RT-PCR法更灵敏。表明初步建立了检测IHNV的液相芯片技术，为进一步构建其他水生动物病原体快速高通量检测平台提供参考。
용DNAStar7.0연건대GenBank중IHNV적N기인진행서렬분석，설계IHNV특이성인물병표기생물소，장탐침안기화수식，여형광편마미구우련후여RT-PCR산물잡교반응，용액상심편의기검측형광신호，건립료전염성조혈기관배사병독（IHNV）적액상심편쾌속검측기술。결과표명액상심편검측체계대IHNV병독핵산적최저검출량위100pg，차특이성고，여기타병독무교차반응。응용건립적액상심편기술검측어류중IHNV，병여RT-PCR방법진행비교，검측결과기본일치，단해방법비RT-PCR법경령민。표명초보건립료검측IHNV적액상심편기술，위진일보구건기타수생동물병원체쾌속고통량검측평태제공삼고。
A liquid chip assay was developed to detect infectious haematopoietic necrosis virus(IHNV). The IHNV N gene in the GenBank was analyzed using the software DNAStar 7.0. Specific IHNV primers labeled with biotin was prepared and coupled with fluorescence-coded microspheres. The amino modified probe was used for hybridization with PCR products of IHNV,then fluorescence signal in the reaction system was detected using BD FACS Array. The results showed that IHNV could be accurately detected by the developed liquid chip assay. Its detection limit was 100 pg viral RNA and its specificity was very high without cross reaction with other viruses.The IHNV liquid chip assay was used to detect IHNV in fish samples and compared with the conventional RT-PCR resulting in basically the same,but more sensitive than RT-PCR. The development of IHNV liquid chip assay provided references for further research on rapidly high throughput detection for other aquatic pathogens with the technique.