CAJ | 학술논문

[目的]分别以鸡和鸭红细胞（RBCs）作指示剂，检测鸭体内高致病性禽流感病毒血凝抑制（HI）抗体结果的一致性。[方法]在对360份鸭血清进行H5亚型高致病性禽流感病毒（Re-6株）HI抗体检测时，分别使用1%鸡红细胞悬液和1%鸭红细胞悬液做指示剂，观察结果，计算阳性率、阳性一致性、阴性一致性、符合率，并进行Kappa检验。[结果]1%鸭红细胞悬液作指示剂的阳性率（76.7%）显著高于鸡的（5.8%），且有统计学意义（p<0.01）。两种方法共同检出13份抗体阳性和76份阴性，阳性一致性为61.9%（95%置信区间：41.1%–82.7%），阴性一致性为22.4%（95%置信区间：18.0%–26.9%），符合率24.7%（95%置信区间：20.3%–29.2%），Kappa值为-0.023（95%置信区间：-0.051–0.004）。[结论]2种红细胞作指示剂的检测结果阳性率有显著差异，结果一致性强度为极差。
[목적]분별이계화압홍세포（RBCs）작지시제，검측압체내고치병성금류감병독혈응억제（HI）항체결과적일치성。[방법]재대360빈압혈청진행H5아형고치병성금류감병독（Re-6주）HI항체검측시，분별사용1%계홍세포현액화1%압홍세포현액주지시제，관찰결과，계산양성솔、양성일치성、음성일치성、부합솔，병진행Kappa검험。[결과]1%압홍세포현액작지시제적양성솔（76.7%）현저고우계적（5.8%），차유통계학의의（p<0.01）。량충방법공동검출13빈항체양성화76빈음성，양성일치성위61.9%（95%치신구간：41.1%–82.7%），음성일치성위22.4%（95%치신구간：18.0%–26.9%），부합솔24.7%（95%치신구간：20.3%–29.2%），Kappa치위-0.023（95%치신구간：-0.051–0.004）。[결론]2충홍세포작지시제적검측결과양성솔유현저차이，결과일치성강도위겁차。
To compare the consistency of the results of antibody against highly pathogenic avian influenza virus(HPAIV) in ducks by hemagglutination inhibition test with chicken or duck red blood cell(RBC) as indicators,360 duck se-rum samples were tested for H5 subtype HPAIV(Re-6 strain) antibody by HI test with 1% chicken and duck RBCs as indicators respectively. Test results were observed,the positive rate,the positive and negative consistence,the coinci-dence rate were calculated,and consistence was determined by Kappa test. The positive rate(76.7%) of HI antibody with 1% duck RBC was higher than that with chicken RBC(5.8%),suggesting a statistically significant difference (p<0.01). 13 positive and 76 negative were detected with the two indicators together with a positive consistence of 61.9%(95%CI:741.1%–82.7%) and a negative consistence of 22.4%(95% CI:18.0%–26.9%),and a coincidence of 24.7%(95%CI: 20.3%–29.2%).The Kappa value was -0.023(95%CI: -0.051–0.004). The results showed that there was a significant difference between the two positive rates of the HI tests with chicken or duck RBC as indica-tors,and a poor consistency between them.