CAJ | 학술논문

目的：探讨缺氧预处理(HPC)对创伤性脑损伤(TBI)大鼠挫伤灶周围皮层组织中缺氧诱导因子-1α(HIF-1α)、葡萄糖转运体3型(GLUT-3)表达及神经元存活影响。方法120只Sprague-Dawley大鼠按随机数字法分为对照组(12只)、TBI组(54只)、HPCT组(54只)。TBI组按Feeney自由落体撞击法建立大鼠TBI模型，HPCT组先给予3 d HPC(50.47 kPa，3 d，3 h/d)，之后同法致伤。采用RT-PCR及Western blotting检测伤后1、4、8、12 h及1、3、7、14 d挫伤灶周围HIF-1α、GLUT-3 mRNA及蛋白的表达，并分析HIF-1α与GLUT-3之间的相关性，采用免疫组化检测伤后14 d挫伤灶周围神经元核蛋白(NeuN)阳性表达率来观察神经元存活情况。多组间比较行单因素方差分析，用LSD法行两两比较分析2组间差异，相关性分析用Pearson相关分析，以P<0.05为差异具有统计学意义。结果 TBI组伤后4 h至3 d HIF-1α、GLUT-3的表达均明显增加(P<0.05)。HPCT组HIF-1α及GLUT-3的表达在伤后1 h即增强，伤后4 h至7 d HIF-1α、GLUT-3表达量和伤后14 d NeuN阳性细胞数均明显高于TBI组，差异均具有统计学意义(P<0.05)。相关性分析表明HIF-1α与GLUT-3 mRNA及蛋白的表达均呈正相关。结论 HPC可通过诱导皮层脑组织HIF-1α的表达，上调GLUT-3 mRNA及蛋白的表达，进而提高TBI后急性期神经元的存活率。
목적：탐토결양예처리(HPC)대창상성뇌손상(TBI)대서좌상조주위피층조직중결양유도인자-1α(HIF-1α)、포도당전운체3형(GLUT-3)표체급신경원존활영향。방법120지Sprague-Dawley대서안수궤수자법분위대조조(12지)、TBI조(54지)、HPCT조(54지)。TBI조안Feeney자유락체당격법건립대서TBI모형，HPCT조선급여3 d HPC(50.47 kPa，3 d，3 h/d)，지후동법치상。채용RT-PCR급Western blotting검측상후1、4、8、12 h급1、3、7、14 d좌상조주위HIF-1α、GLUT-3 mRNA급단백적표체，병분석HIF-1α여GLUT-3지간적상관성，채용면역조화검측상후14 d좌상조주위신경원핵단백(NeuN)양성표체솔래관찰신경원존활정황。다조간비교행단인소방차분석，용LSD법행량량비교분석2조간차이，상관성분석용Pearson상관분석，이P<0.05위차이구유통계학의의。결과 TBI조상후4 h지3 d HIF-1α、GLUT-3적표체균명현증가(P<0.05)。HPCT조HIF-1α급GLUT-3적표체재상후1 h즉증강，상후4 h지7 d HIF-1α、GLUT-3표체량화상후14 d NeuN양성세포수균명현고우TBI조，차이균구유통계학의의(P<0.05)。상관성분석표명HIF-1α여GLUT-3 mRNA급단백적표체균정정상관。결론 HPC가통과유도피층뇌조직HIF-1α적표체，상조GLUT-3 mRNA급단백적표체，진이제고TBI후급성기신경원적존활솔。
Objective To investigate the effect of hypoxic preconditioning (HPC) on the expression of hypoxia-inducible factor-1α, glucose transporter-3 and neuronal survival in cerebral cortex in rats with traumatic brain injury (TBI). Methods A total of 120 SD rats were randomly assigned to control group (CON, n=12), traumatic brain injury group (TBI, n=54) and traumatic brain injury after HPC group (HPCT, n=54). The rats of TBI group were established the TBI model by Feeney free falling dart impact method;The rats of HPCT group were treated with HPC (50.47 kPa, 3 h/d, 3 d) firstly, and then establish the HPCT model by Feeney free falling dart impact method. Detect the expression of HIF-1α、GLUT-3 mRNA and protein around the contusion focus on 1 h, 4 h, 8 h, 12 h, 1 d, 3 d, 7 d, 14 d post-injury by RT-PCR and Western blotting, analysis the relevance between HIF-1αand GLUT-3, observe the neuronal survival situation of NeuN positive expression rate around the contusion focus by immunohistochemistry in 14 d post-injury. Take single factor analysis of variance among groups, analyzed by multiple comparisons with LSD method, correlation analysis with Pearson correlation analysis method, set P<0.05 as the statistically significant difference. Results TBI group, both HIF-1αand GLUT-3 increased expression significantly on 4 h, 8 h, 12 h, 1 d and 3 d post-injury (P<0.05). HPCT group, both HIF-1αand GLUT-3 increased expression on 1h post-injury, the expression of HIF-1αand GLUT-3 on 4 h to 7 d post-injury and the count of NeuN positive cells 14 d post-injury were both significantly higher than TBI group (P<0.05). Correlation analysis showed that the expression of HIF-1αand GLUT-3 mRNA and protein were positive correlated. Conclusion HPC can induce the expression of HIF-1αin the cortical brain tissue, raise the expression of GLUT-3 mRNA and protein, and improve the acute neuronal survival rate post-injury of TBI.