CAJ | 학술논문

目的：制备胶原-纤维蛋白胶双层复合材料，评估其作为皮肤组织工程材料复合人脐带间充质干细胞（hUCMSC）修复小鼠全层皮肤创伤的效果。方法制备胶原-纤维蛋白双层材料，扫描电镜检测双层支架材料表面结构。24只小鼠建立全层皮肤创面模型后，随机分为3组：空白组用无菌纱布处理伤口、hUCMSC 复合胶原组和 hUCMSC 复合胶原-纤维蛋白胶组，分别采用各组敷料处理创面。治疗后5、10、15 d，观察创面愈合情况，计算3组创面愈合率并进行对比。治疗后 15 d，取小鼠创面新生皮肤组织苏木精-伊红染色，观察新生皮肤组织结构。结果扫描电镜结果显示：双层材料胶原层横断面呈现出高孔径结构并且互相连通，纤维蛋白胶层横断面显示紧密的三维网状结构；创面愈合结果显示：hUCMSC 复合胶原－纤维蛋白胶组治疗5、10、15 d 创面愈合率分别达到（62．62±6．27）％、（88．47±7．14）％、（96．14 ±4．56）％，优于空白组（t ＝17．03、16．22、15．69，P 均＜0．01），同时也优于 hUCMSC 复合胶原组（t ＝2．71、5．60、5．28，P 均＜0．05）。hUCMSC 复合胶原-纤维蛋白胶组组织学检查新生皮肤组织结构良好。结论胶原-纤维蛋白胶双层支架具有较好的三维空间结构，复合 hUCMSC 后有利于促进皮肤创面愈合。
목적：제비효원-섬유단백효쌍층복합재료，평고기작위피부조직공정재료복합인제대간충질간세포（hUCMSC）수복소서전층피부창상적효과。방법제비효원-섬유단백쌍층재료，소묘전경검측쌍층지가재료표면결구。24지소서건립전층피부창면모형후，수궤분위3조：공백조용무균사포처리상구、hUCMSC 복합효원조화 hUCMSC 복합효원-섬유단백효조，분별채용각조부료처리창면。치료후5、10、15 d，관찰창면유합정황，계산3조창면유합솔병진행대비。치료후 15 d，취소서창면신생피부조직소목정-이홍염색，관찰신생피부조직결구。결과소묘전경결과현시：쌍층재료효원층횡단면정현출고공경결구병차호상련통，섬유단백효층횡단면현시긴밀적삼유망상결구；창면유합결과현시：hUCMSC 복합효원－섬유단백효조치료5、10、15 d 창면유합솔분별체도（62．62±6．27）％、（88．47±7．14）％、（96．14 ±4．56）％，우우공백조（t ＝17．03、16．22、15．69，P 균＜0．01），동시야우우 hUCMSC 복합효원조（t ＝2．71、5．60、5．28，P 균＜0．05）。hUCMSC 복합효원-섬유단백효조조직학검사신생피부조직결구량호。결론효원-섬유단백효쌍층지가구유교호적삼유공간결구，복합 hUCMSC 후유리우촉진피부창면유합。
Objective Preparation of collagen-fibrin double layer membrane.To study the effect of human umbilical cord mesenchymal stem cell (hUCMSC) combined with collagen-fibrin double layer membrane on wound healing in mice.Methods Preparation of collagen-fibrin double layer membrane.The surface stucture of the double loyer membrane was investigated by SEM.Twenty-four mouse were prepaird as full thickness skin wound model and randomly divided into 3 groups:control group was bound up their wounds by conventional method,hUCMSC combined with collagen membrane treated group and hUCMSC combined with collagen-fibrin double layer membrane treated group.Results The SEMresults showed that <br> collagen-layer exhibited a highly porous and interconnected structure.The wound healing rate in double layer membrane combined with hUCMSC treated group at 5,1 0,1 5 day was (62.62 ±6.27)%,(88.47 ± 7.1 4)%,(96.1 4 ±4.56)%,better than control group (t =1 7.03,1 6.22,1 5.69,P <0.01 ),and hUCMSC combined with collagen membrane treated group (t =2.71 ,5.60,5.28,P <0.05),differences were significant.Conclusions Collagen-fibrin double layer membrane showed a good three dimensional space structure,combined with hUCMSC could accelerate wound healing.