中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
10期
749-752
,共4页
眼疾病%泪液%蛋白质谱%溶菌酶%乳铁蛋白
眼疾病%淚液%蛋白質譜%溶菌酶%乳鐵蛋白
안질병%루액%단백질보%용균매%유철단백
Eye diseases%Tears%Protein spectrum%Lysozyme%Lactoferrin
目的 比较甲状腺相关性眼病(TAO)患者与健康人泪液中的溶菌酶C和乳铁蛋白的表达差异.方法 泪液样品采自TAO活跃期的患者25眼(病例组)和年龄、性别相匹配的健康志愿者25眼(对照组).将泪液标本进行十二烷基硫酸钠-聚丙烯酰胺(SDS-PAGE)凝胶电泳,采用15％的凝胶分离两组样品的差异蛋白条带,收集标记的差异蛋白条带进行凝胶内胰蛋白酶消化,然后进行质谱分析,以确定在差异条带中的蛋白质组分.Scaffold搜索引擎分析质谱的结果,进行蛋白质的鉴定.结果 通过对差异蛋白条带的质谱分析,发现在TAO患者泪液中大多数蛋白表现为下调或者缺失,但是溶菌酶C和乳铁蛋白却发生了上调.鉴定蛋白的相对定量比值(病例/对照):溶菌酶C为4.88,乳铁蛋白为1.60.结论 TAO患者泪液中的溶菌酶C和乳铁蛋白均发生上调,说明两者可能参与了TAO的炎症过程.
目的 比較甲狀腺相關性眼病(TAO)患者與健康人淚液中的溶菌酶C和乳鐵蛋白的錶達差異.方法 淚液樣品採自TAO活躍期的患者25眼(病例組)和年齡、性彆相匹配的健康誌願者25眼(對照組).將淚液標本進行十二烷基硫痠鈉-聚丙烯酰胺(SDS-PAGE)凝膠電泳,採用15％的凝膠分離兩組樣品的差異蛋白條帶,收集標記的差異蛋白條帶進行凝膠內胰蛋白酶消化,然後進行質譜分析,以確定在差異條帶中的蛋白質組分.Scaffold搜索引擎分析質譜的結果,進行蛋白質的鑒定.結果 通過對差異蛋白條帶的質譜分析,髮現在TAO患者淚液中大多數蛋白錶現為下調或者缺失,但是溶菌酶C和乳鐵蛋白卻髮生瞭上調.鑒定蛋白的相對定量比值(病例/對照):溶菌酶C為4.88,乳鐵蛋白為1.60.結論 TAO患者淚液中的溶菌酶C和乳鐵蛋白均髮生上調,說明兩者可能參與瞭TAO的炎癥過程.
목적 비교갑상선상관성안병(TAO)환자여건강인루액중적용균매C화유철단백적표체차이.방법 루액양품채자TAO활약기적환자25안(병례조)화년령、성별상필배적건강지원자25안(대조조).장루액표본진행십이완기류산납-취병희선알(SDS-PAGE)응효전영,채용15％적응효분리량조양품적차이단백조대,수집표기적차이단백조대진행응효내이단백매소화,연후진행질보분석,이학정재차이조대중적단백질조분.Scaffold수색인경분석질보적결과,진행단백질적감정.결과 통과대차이단백조대적질보분석,발현재TAO환자루액중대다수단백표현위하조혹자결실,단시용균매C화유철단백각발생료상조.감정단백적상대정량비치(병례/대조):용균매C위4.88,유철단백위1.60.결론 TAO환자루액중적용균매C화유철단백균발생상조,설명량자가능삼여료TAO적염증과정.
Objective To explore the differential expressions of lysozyme C and lactoferrin in tears of thyroid-associated ophthalmopathy (TAO) patients versus healthy subjects by proteomics.Methods Tear samples were obtained from patients with active period TAO and age and gender-matched healthy subjects without symptoms of ocular surface.Then they were divided into patient and control groups.Then tear samples of two groups were analyzed.sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) of 15％ gel was performed to determine the different protein bands in sample groups.And different labeled protein bands were collected for in-gel tryptic digestion.Mass spectrometry was employed to determine the protein components from different protein bands.Then Scaffold search engine was used for analyzing the results of mass spectrometry and identifying specific proteins.Results Based on mass spectrometric analysis of different protein bands,most proteins were down-regulated or became absent in TAO patients.Both lysozyme C and lactoferrin were up-regulated.Identification of protein relative quantitative ratio (patient/control):lysozyme C:4.88,lactoferrin:1.61.Conclusions Lysozyme C and lactoferrin are two important effectors of tear function and metabolism.Both are up-regulated in TAO patients' tears.Thus both are probably involved in inflammatory process of TAO and play synergistic roles in the pathogenesis of disease.
