CAJ | 학술논문

目的：研究肺结核患者外周血单个核细胞(PBMCs)经结核特异性抗原刺激后中性粒细胞激活蛋白3(NAP3)的表达情况并与结核潜伏感染(latent tuberculosis infection,LTBI)者及非结核感染健康对照者进行比较。方法提取6例活动期肺结核患者和6例 LTBI 个体 PBMCs,用结核分枝杆菌特异性抗原肽刺激后收集细胞,采用基因芯片分析方法比较两组 NAP3表达情况。然后提取60例活动期肺结核患者和60名 PPD 阳性健康个体(根据γ干扰素表达情况将其分成 LTBI 组和非结核感染健康对照组)的 PBMCs,用结核分枝杆菌特异性抗原肽刺激后,采用酶联免疫吸附试验(ELISA)定量检测刺激前、后细胞培养液上清中 NAP3的浓度。结果基因芯片分析发现活动期肺结核患者 NAP3表达明显高于 LTBI 组(U =1.000,P =0.0043)。ELISA 分析结果发现特异性抗原刺激前细胞培养液上清中3组的 NAP3浓度差异无统计学意义(F =0.7341,P =0.4821),而刺激后只有活动期肺结核组和非结核感染健康对照组之间的 NAP3浓度相比差异有统计学意义(P =0.002)。结论高表达的 NAP3可作为诊断结核的指标之一,但是无法区分潜伏感染和活动期感染病例。
목적：연구폐결핵환자외주혈단개핵세포(PBMCs)경결핵특이성항원자격후중성립세포격활단백3(NAP3)적표체정황병여결핵잠복감염(latent tuberculosis infection,LTBI)자급비결핵감염건강대조자진행비교。방법제취6례활동기폐결핵환자화6례 LTBI 개체 PBMCs,용결핵분지간균특이성항원태자격후수집세포,채용기인심편분석방법비교량조 NAP3표체정황。연후제취60례활동기폐결핵환자화60명 PPD 양성건강개체(근거γ간우소표체정황장기분성 LTBI 조화비결핵감염건강대조조)적 PBMCs,용결핵분지간균특이성항원태자격후,채용매련면역흡부시험(ELISA)정량검측자격전、후세포배양액상청중 NAP3적농도。결과기인심편분석발현활동기폐결핵환자 NAP3표체명현고우 LTBI 조(U =1.000,P =0.0043)。ELISA 분석결과발현특이성항원자격전세포배양액상청중3조적 NAP3농도차이무통계학의의(F =0.7341,P =0.4821),이자격후지유활동기폐결핵조화비결핵감염건강대조조지간적 NAP3농도상비차이유통계학의의(P =0.002)。결론고표체적 NAP3가작위진단결핵적지표지일,단시무법구분잠복감염화활동기감염병례。
Objective To compare the neutrophil activating protein 3 (NAP3 )expression from peripheral blood mononuclear cells (PBMCs ) stimulated with Mycobacterium tuberculosis-specific antigens in pulmonary tuberculosis patients with latent tuberculosis infection (LTBI)and non-tuberculosis infection healthy controls.Methods The NAP3 level in PBMCs stimulated with Mycobacterium tuberculosis-specific antigens from six active tuberculosis patients and six LTBI controls was compared using microarray analysis method.Then the concentration of NAP3 in culture supernatants of PBMCs stimulated with Mycobacterium tuberculosis-specific antigens from 60 active tuberculosis patients and 60 PPD-positive healthy controls (divided into LTBI group and non-tuberculous infection healthy control group based on the expression of interferon-γ) was quantitatively detected by enzyme-linked immunosorbent assay (ELISA).Results Microarray analysis showed that NAP3 level in patients with active tuberculosis was significantly higher than that in LTBI group (U = 1.000,P =0.004 3).ELISA analysis revealed there was no difference in NAP3 concentration in PBMCs culture supernatants before stimulation among the three groups (F =0.734 1,P =0.482 1),but there was significant difference in NAP3 concentration after stimulation between active tuberculosis group and non-tuberculous infection healthy control group (P =0.002).Conclusions High expression of NAP3 can be used as a biomarker for diagnosis of tuberculosis,but latent infection and active infection can not be distinguished.