CAJ | 학술논문

目的：观察红霉素对4-羟基壬烯醛(4-HNE)刺激的气道上皮细胞基质金属蛋白酶2(MMP-2)、MMP-9、环氧合酶2(COX-2)和活化蛋白-2(AP-2)表达的影响。方法 Western blot 检测红霉素(5μmol/L)预孵育不同时间(24、36、48 h)后4-HNE(10μmol/L 作用4 h)刺激的气道上皮细胞MMP-2、MMP-9、COX-2和 AP-2蛋白表达的变化。结果红霉素预孵育36 h 和48 h 后明显抑制4-HNE刺激的气道上皮细胞 MMP-2、MMP-9、COX-2合成，明显抑制4-HNE 刺激的气道上皮细胞核转录因子 AP-2的激活，与红霉素未预孵育组相比，差异具有统计学意义(P <0.05)。结论红霉素可明显抑制4-HNE 刺激的气道上皮细胞 MMP-2、MMP-9、COX-2表达，其机制可能和抑制核转录因子 AP-2的激活有关。
목적：관찰홍매소대4-간기임희철(4-HNE)자격적기도상피세포기질금속단백매2(MMP-2)、MMP-9、배양합매2(COX-2)화활화단백-2(AP-2)표체적영향。방법 Western blot 검측홍매소(5μmol/L)예부육불동시간(24、36、48 h)후4-HNE(10μmol/L 작용4 h)자격적기도상피세포MMP-2、MMP-9、COX-2화 AP-2단백표체적변화。결과홍매소예부육36 h 화48 h 후명현억제4-HNE자격적기도상피세포 MMP-2、MMP-9、COX-2합성，명현억제4-HNE 자격적기도상피세포핵전록인자 AP-2적격활，여홍매소미예부육조상비，차이구유통계학의의(P <0.05)。결론홍매소가명현억제4-HNE 자격적기도상피세포 MMP-2、MMP-9、COX-2표체，기궤제가능화억제핵전록인자 AP-2적격활유관。
Objective To observe the effects of erythromycin on the expression of matrix metalloproteinase-2 (MMP-2 ),MMP-9,cyclooxygenase-2 (COX-2 )and activator protein-2 (AP-2 )in airway epithelial cells induced by 4-hydroxy-2-nonenal (4-HNE).Methods Airway epithelial cells were preincubated with 5 μmol/L erythromycin for different time (24,36,48 hours )and incubated with 10 μmol/L 4-HNE for four hours.The protein expressions of MMP-2,MMP-9,COX-2,and AP-2 were detected by Western blot.Results Preincubation with 5 μmol/L erythromycin for 36 hours and 48 hours significantly inhibited the expressions of MMP-2,MMP-9,COX-2,and AP-2 in airway epithelial cells induced by 4-HNE.Compared with the control group,the difference was significant (P < 0.05 ). Conclusions Erythromycin could inhibit the expressions of MMP-2,MMP-9,and COX-2 in airway epithelial cells induced by 4-HNE,which may be associated with inhibiting the stimulation of AP-2.