CAJ | 학술논문

研究了长春七根部挥发油的提取方法，结合该部位的抑菌活性测试，提高挥发油的综合利用价值。采用挥发器测定和索式抽提两种方法和不同溶剂分别对长春七根部进行挥发油的提取，通过GC-MS联用技术测定分析有效成分，并进行比对，同时测试了其对大肠杆菌和金黄色葡萄球菌的抑菌活性。结果表明，两种方法的提取率和提取成分的种类与含量存在差异。抑菌活性测试表明长春七根部挥发油对大肠杆菌和金黄色葡萄球菌均能产生明显的抑制作用。两种方法可以互补地用于长春七根部中的有效成分的提取，结合挥发油的抑菌活性，进一步明确了该部位的应用前景。
연구료장춘칠근부휘발유적제취방법，결합해부위적억균활성측시，제고휘발유적종합이용개치。채용휘발기측정화색식추제량충방법화불동용제분별대장춘칠근부진행휘발유적제취，통과GC-MS련용기술측정분석유효성분，병진행비대，동시측시료기대대장간균화금황색포도구균적억균활성。결과표명，량충방법적제취솔화제취성분적충류여함량존재차이。억균활성측시표명장춘칠근부휘발유대대장간균화금황색포도구균균능산생명현적억제작용。량충방법가이호보지용우장춘칠근부중적유효성분적제취，결합휘발유적억균활성，진일보명학료해부위적응용전경。
The aim of this article is to improve comprehensive value of essential oils through studying the extraction methods of essential oils from roots of Libanotis buchtormensis(Fisch)DC and the antibacterial activity test. The essential oils are extracted by steam distillation and soxhlet extraction with different solvents. The chemical constituents of essential oils are identified and analyzed by GC-MS. The colon bacillus and staphylococcus aurous are used for antibacterial activity test. There are differences in extraction rate,kinds and contents with the different methods and different solvents. The anti-bacterial activity test proves that propagate frequency of colon bacillus and staphylococcus aurous are obviously restrained by essential oils from roots of Libanotis buchtormensis( Fisch) DC. Many compounds are isolated and identified for the first time. Effective constituents can be extracted by two methods in the general. Combining with the antibacterial activity test, essential oils from roots of Libanotis buchtormensis( Fisch) DC will get a very wide application foreground.