中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2015年
10期
42-42,43
,共2页
何广俊%李诗慧%王利胜%卢映%李周
何廣俊%李詩慧%王利勝%盧映%李週
하엄준%리시혜%왕리성%로영%리주
通经活络喷雾剂%质量标准%丹参酮ⅡA%高效液相色谱法
通經活絡噴霧劑%質量標準%丹參酮ⅡA%高效液相色譜法
통경활락분무제%질량표준%단삼동ⅡA%고효액상색보법
Tongjinghuoluo spray%Quality standard%TanshinoneⅡA%HPLC
目的：建立高效液相色谱测定通经活络喷雾剂中丹参酮ⅡA含量的方法。方法利用Diamonsil C18柱色谱柱，流动相为乙腈-水（75∶25）；检测波长为270 nm；流速1.0 mL/min；柱温：25℃。结果丹参酮ⅡA在浓度0.12~6μg/mL范围内呈良好的线性关系,回归方程为：Y=102959X-4680，r=0.9997，平均回收率为99.95%，RSD值为2.49%（n=6）。结论该方法简便，准确可靠，灵敏度高，可用于通经活络喷雾剂的质量控制。
目的：建立高效液相色譜測定通經活絡噴霧劑中丹參酮ⅡA含量的方法。方法利用Diamonsil C18柱色譜柱，流動相為乙腈-水（75∶25）；檢測波長為270 nm；流速1.0 mL/min；柱溫：25℃。結果丹參酮ⅡA在濃度0.12~6μg/mL範圍內呈良好的線性關繫,迴歸方程為：Y=102959X-4680，r=0.9997，平均迴收率為99.95%，RSD值為2.49%（n=6）。結論該方法簡便，準確可靠，靈敏度高，可用于通經活絡噴霧劑的質量控製。
목적：건립고효액상색보측정통경활락분무제중단삼동ⅡA함량적방법。방법이용Diamonsil C18주색보주，류동상위을정-수（75∶25）；검측파장위270 nm；류속1.0 mL/min；주온：25℃。결과단삼동ⅡA재농도0.12~6μg/mL범위내정량호적선성관계,회귀방정위：Y=102959X-4680，r=0.9997，평균회수솔위99.95%，RSD치위2.49%（n=6）。결론해방법간편，준학가고，령민도고，가용우통경활락분무제적질량공제。
Objective The purpose of the study is to establish a high performance liquid chromatography(HPLC) method to measure the content of tanshinoneⅡA in Tongjinghuoluo spray.Methods Diamonsil C18 column was used; the mobile phase of HPLC was acetonitrile-water(75∶25); the detection wavelength was 270 nm; lfow rate was 1 mL/min; column temperature was 25℃.Results TanshinoneⅡA in the concentration 0.12-6 mL/min showed a good linear relation and the regression equation is Y=102959X-4680, correlation coefifcient is 0.9997, the average recovery was 99.95%, RSD is 2.49%(n=6).Conclusion The method is simple, accurate and reliable with high sensitivity and can be used for the Tongjinghuoluo spray quality control.
