山东体育学院学报
山東體育學院學報
산동체육학원학보
JOURNAL OF SHANDONG PHYSICAL EDUCATION INSTITUTE
2015年
2期
68-73
,共6页
中枢疲劳%运动性疲劳%大鼠%海马组织%胶质细胞源性神经营养因子%胶质细胞源性神经营养因子受体
中樞疲勞%運動性疲勞%大鼠%海馬組織%膠質細胞源性神經營養因子%膠質細胞源性神經營養因子受體
중추피로%운동성피로%대서%해마조직%효질세포원성신경영양인자%효질세포원성신경영양인자수체
central fatigue%exercise -induced fatigue%rat%hippocampus%glial cell line -derived neurotrophic factor (GDNF)%GDNF family receptor alpha -1 (GFRα-1)
目的:探讨大鼠海马组织胶质细胞源性神经营养因子(glial cell line -derived neurotro-phic factor,GDNF)、GDNF 家族受体α-1信使 RNA (GDNF?family receptor α-1mRNA, GFRα-1mRNA)及其蛋白表达在中枢疲劳后恢复期不同时相的动态变化特征。方法:雄性2月龄 SD 大鼠32只,随机分为对照组(C 组,n =8)、实验组(E 组,n =24);E 组进行7周疲劳模型运动后,又随机分为运动后即刻组(E0组,n =8)、恢复期12 h 组(E 1组,n =8)、恢复期24 h组(E2组,n =8)。C 组正常笼养,E 组进行7周递增负荷跑台训练。经7周疲劳模型运动后即刻,C 组和 E0组腹腔注射10%水合氯醛糖浆(0.3mL/100g)麻醉后取海马组织,用于测定5-羟色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、GDNF、GFRα-1mRNA 及其蛋白表达;E1组和 E2组分别在恢复期12 h、24 h 做上述同样取材和测试。结果:1)大鼠经7周递增负荷跑台训练后即刻,海马组织5-HT 升高(P <0.01),DA 下降(P <0.01),DA /5-HT 下降(P <0.05)。2)运动后即刻海马组织 GDNF、GFRα-1mRNA 相对表达率和 GDNF、GFRα-1平均蛋白表达水平升高;恢复期12 h 高于 C 组和 E0组(P <0.01);恢复期24 h 降低并低于 E1组(P <0.01)。结论:1)经7周疲劳模型运动后大鼠已经出现中枢疲劳。2)运动性中枢疲劳后大鼠海马组织 GDNF、GFRα-1mRNA 及其蛋白表达水平上升,提示 GDNF 和GFRα-1可能参与了中枢疲劳恢复的神经生物学调控过程。
目的:探討大鼠海馬組織膠質細胞源性神經營養因子(glial cell line -derived neurotro-phic factor,GDNF)、GDNF 傢族受體α-1信使 RNA (GDNF?family receptor α-1mRNA, GFRα-1mRNA)及其蛋白錶達在中樞疲勞後恢複期不同時相的動態變化特徵。方法:雄性2月齡 SD 大鼠32隻,隨機分為對照組(C 組,n =8)、實驗組(E 組,n =24);E 組進行7週疲勞模型運動後,又隨機分為運動後即刻組(E0組,n =8)、恢複期12 h 組(E 1組,n =8)、恢複期24 h組(E2組,n =8)。C 組正常籠養,E 組進行7週遞增負荷跑檯訓練。經7週疲勞模型運動後即刻,C 組和 E0組腹腔註射10%水閤氯醛糖漿(0.3mL/100g)痳醉後取海馬組織,用于測定5-羥色胺(5-hydroxytryptamine,5-HT)、多巴胺(dopamine,DA)、GDNF、GFRα-1mRNA 及其蛋白錶達;E1組和 E2組分彆在恢複期12 h、24 h 做上述同樣取材和測試。結果:1)大鼠經7週遞增負荷跑檯訓練後即刻,海馬組織5-HT 升高(P <0.01),DA 下降(P <0.01),DA /5-HT 下降(P <0.05)。2)運動後即刻海馬組織 GDNF、GFRα-1mRNA 相對錶達率和 GDNF、GFRα-1平均蛋白錶達水平升高;恢複期12 h 高于 C 組和 E0組(P <0.01);恢複期24 h 降低併低于 E1組(P <0.01)。結論:1)經7週疲勞模型運動後大鼠已經齣現中樞疲勞。2)運動性中樞疲勞後大鼠海馬組織 GDNF、GFRα-1mRNA 及其蛋白錶達水平上升,提示 GDNF 和GFRα-1可能參與瞭中樞疲勞恢複的神經生物學調控過程。
목적:탐토대서해마조직효질세포원성신경영양인자(glial cell line -derived neurotro-phic factor,GDNF)、GDNF 가족수체α-1신사 RNA (GDNF?family receptor α-1mRNA, GFRα-1mRNA)급기단백표체재중추피로후회복기불동시상적동태변화특정。방법:웅성2월령 SD 대서32지,수궤분위대조조(C 조,n =8)、실험조(E 조,n =24);E 조진행7주피로모형운동후,우수궤분위운동후즉각조(E0조,n =8)、회복기12 h 조(E 1조,n =8)、회복기24 h조(E2조,n =8)。C 조정상롱양,E 조진행7주체증부하포태훈련。경7주피로모형운동후즉각,C 조화 E0조복강주사10%수합록철당장(0.3mL/100g)마취후취해마조직,용우측정5-간색알(5-hydroxytryptamine,5-HT)、다파알(dopamine,DA)、GDNF、GFRα-1mRNA 급기단백표체;E1조화 E2조분별재회복기12 h、24 h 주상술동양취재화측시。결과:1)대서경7주체증부하포태훈련후즉각,해마조직5-HT 승고(P <0.01),DA 하강(P <0.01),DA /5-HT 하강(P <0.05)。2)운동후즉각해마조직 GDNF、GFRα-1mRNA 상대표체솔화 GDNF、GFRα-1평균단백표체수평승고;회복기12 h 고우 C 조화 E0조(P <0.01);회복기24 h 강저병저우 E1조(P <0.01)。결론:1)경7주피로모형운동후대서이경출현중추피로。2)운동성중추피로후대서해마조직 GDNF、GFRα-1mRNA 급기단백표체수평상승,제시 GDNF 화GFRα-1가능삼여료중추피로회복적신경생물학조공과정。
Objective:To investigate the dynamic changing features of the glial cell line -derived neu-rotrophic factor (GDNF),GDNF family receptor α-1mRNA (GFRα-1mRNA)and relative pro-tein expression in hippocampus of rats during different phases of recovery period after fatigue.Meth-ods:32 male SD rats (2 month old)were randomly divided into control group (C group,n =8)and experimental groups (E group,n =24).At the sampling time,the experimental groups were ran-domly divided into the group immediately (E0,n =8),12 h (E1,n =8),and 24 h (E2,n =8) after exhaustive running.All rats in experimental groups were assigned a 7 -week training program increasing load by degrees with a final exhaustive running.The 5 -HT,DA,and the expressions of GDNF and GFRα-1mRNA from hippocampus of rats were measured.Results:All rats in experimen-tal groups were assigned a 7 -week training program increasing load by degrees.Immediately after the last exhaustive running:1)the hippocampus 5 -HT was significantly higher(P <0.05),and DA,DA /5 -HT were significantly lower (P <0.01)than those of C group.2)The correspondence expression rates of GDNF and GFRα-1mRNA,and the protein expressions of GDNF and GFRα-1 were higher than those of C respectively.12h post -exercise,all indexes of E1 group were higher than those of C and E0,respectively (P <0.01 ).24 h post -exercise,all indexes of E2 group were lower than those of E1(P <0.01).Conclusion:1)The rat was instantly reach central fatigue after treadmill movement after 7 weeks.2)While exercise -induced central fatigue occurs,there are up -regulations in the levels of expression of GDNF,GFRα-1mRNA and protein in rat hippo-campus,which indicates that both GDNF and GFRα-1mRNA have participated in neurobiological regulation process recovered from exercise fatigue.