CAJ | 학술논문

目的：探讨大鼠海马组织胶质细胞源性神经营养因子（glial cell line －derived neurotro-phic factor，GDNF）、GDNF 家族受体α－1信使 RNA （GDNF？family receptor α－1mRNA， GFRα－1mRNA）及其蛋白表达在中枢疲劳后恢复期不同时相的动态变化特征。方法：雄性2月龄 SD 大鼠32只，随机分为对照组（C 组，n ＝8）、实验组（E 组，n ＝24）；E 组进行7周疲劳模型运动后，又随机分为运动后即刻组（E0组，n ＝8）、恢复期12 h 组（E 1组，n ＝8）、恢复期24 h组（E2组，n ＝8）。C 组正常笼养，E 组进行7周递增负荷跑台训练。经7周疲劳模型运动后即刻，C 组和 E0组腹腔注射10％水合氯醛糖浆（0．3mL／100g）麻醉后取海马组织，用于测定5－羟色胺（5－hydroxytryptamine，5－HT）、多巴胺（dopamine，DA）、GDNF、GFRα－1mRNA 及其蛋白表达；E1组和 E2组分别在恢复期12 h、24 h 做上述同样取材和测试。结果：1）大鼠经7周递增负荷跑台训练后即刻，海马组织5－HT 升高（P ＜0．01），DA 下降（P ＜0．01），DA ／5－HT 下降（P ＜0．05）。2）运动后即刻海马组织 GDNF、GFRα－1mRNA 相对表达率和 GDNF、GFRα－1平均蛋白表达水平升高；恢复期12 h 高于 C 组和 E0组（P ＜0．01）；恢复期24 h 降低并低于 E1组（P ＜0．01）。结论：1）经7周疲劳模型运动后大鼠已经出现中枢疲劳。2）运动性中枢疲劳后大鼠海马组织 GDNF、GFRα－1mRNA 及其蛋白表达水平上升，提示 GDNF 和GFRα－1可能参与了中枢疲劳恢复的神经生物学调控过程。
목적：탐토대서해마조직효질세포원성신경영양인자（glial cell line －derived neurotro-phic factor，GDNF）、GDNF 가족수체α－1신사 RNA （GDNF？family receptor α－1mRNA， GFRα－1mRNA）급기단백표체재중추피로후회복기불동시상적동태변화특정。방법：웅성2월령 SD 대서32지，수궤분위대조조（C 조，n ＝8）、실험조（E 조，n ＝24）；E 조진행7주피로모형운동후，우수궤분위운동후즉각조（E0조，n ＝8）、회복기12 h 조（E 1조，n ＝8）、회복기24 h조（E2조，n ＝8）。C 조정상롱양，E 조진행7주체증부하포태훈련。경7주피로모형운동후즉각，C 조화 E0조복강주사10％수합록철당장（0．3mL／100g）마취후취해마조직，용우측정5－간색알（5－hydroxytryptamine，5－HT）、다파알（dopamine，DA）、GDNF、GFRα－1mRNA 급기단백표체；E1조화 E2조분별재회복기12 h、24 h 주상술동양취재화측시。결과：1）대서경7주체증부하포태훈련후즉각，해마조직5－HT 승고（P ＜0．01），DA 하강（P ＜0．01），DA ／5－HT 하강（P ＜0．05）。2）운동후즉각해마조직 GDNF、GFRα－1mRNA 상대표체솔화 GDNF、GFRα－1평균단백표체수평승고；회복기12 h 고우 C 조화 E0조（P ＜0．01）；회복기24 h 강저병저우 E1조（P ＜0．01）。결론：1）경7주피로모형운동후대서이경출현중추피로。2）운동성중추피로후대서해마조직 GDNF、GFRα－1mRNA 급기단백표체수평상승，제시 GDNF 화GFRα－1가능삼여료중추피로회복적신경생물학조공과정。
Objective:To investigate the dynamic changing features of the glial cell line -derived neu-rotrophic factor (GDNF),GDNF family receptor α-1mRNA (GFRα-1mRNA)and relative pro-tein expression in hippocampus of rats during different phases of recovery period after fatigue.Meth-ods:32 male SD rats (2 month old)were randomly divided into control group (C group,n =8)and experimental groups (E group,n =24).At the sampling time,the experimental groups were ran-domly divided into the group immediately (E0,n =8),12 h (E1,n =8),and 24 h (E2,n =8) after exhaustive running.All rats in experimental groups were assigned a 7 -week training program increasing load by degrees with a final exhaustive running.The 5 -HT,DA,and the expressions of GDNF and GFRα-1mRNA from hippocampus of rats were measured.Results:All rats in experimen-tal groups were assigned a 7 -week training program increasing load by degrees.Immediately after the last exhaustive running:1)the hippocampus 5 -HT was significantly higher(P <0.05),and DA,DA /5 -HT were significantly lower (P <0.01)than those of C group.2)The correspondence expression rates of GDNF and GFRα-1mRNA,and the protein expressions of GDNF and GFRα-1 were higher than those of C respectively.12h post -exercise,all indexes of E1 group were higher than those of C and E0,respectively (P <0.01 ).24 h post -exercise,all indexes of E2 group were lower than those of E1(P <0.01).Conclusion:1)The rat was instantly reach central fatigue after treadmill movement after 7 weeks.2)While exercise -induced central fatigue occurs,there are up -regulations in the levels of expression of GDNF,GFRα-1mRNA and protein in rat hippo-campus,which indicates that both GDNF and GFRα-1mRNA have participated in neurobiological regulation process recovered from exercise fatigue.