CAJ | 학술논문

目的：研究骨髓间充质干细胞（ bone marrow mesenchymal stem cell，BMSC）-脱细胞真皮基质（ acellular dermal matrix，ADM）对 SD大鼠皮肤表皮缺损的修复。方法培养 SD 大鼠骨髓细胞，增殖提纯传代至4代时，成骨成脂，流式细胞仪检测鉴定 BMSC，然后将其接种到 ADM，2d 后移植到皮肤表皮缺损的 SD大鼠体内，连续5周观察其修复效果。结果 BMSC-ADM 修复 SD 大鼠皮肤表皮缺损效果良好，5周后，肉眼可见材料与组织结合紧密，有弹性，颜色接近皮肤。结论 BMSC-ADM 可以作为修复SD大鼠皮肤表皮缺损的组织工程材料。
목적：연구골수간충질간세포（ bone marrow mesenchymal stem cell，BMSC）-탈세포진피기질（ acellular dermal matrix，ADM）대 SD대서피부표피결손적수복。방법배양 SD 대서골수세포，증식제순전대지4대시，성골성지，류식세포의검측감정 BMSC，연후장기접충도 ADM，2d 후이식도피부표피결손적 SD대서체내，련속5주관찰기수복효과。결과 BMSC-ADM 수복 SD 대서피부표피결손효과량호，5주후，육안가견재료여조직결합긴밀，유탄성，안색접근피부。결론 BMSC-ADM 가이작위수복SD대서피부표피결손적조직공정재료。
Objective To investigate acellular dermal matrix( ADM ) combined with bone marrow mesenchymal stem cells( BMSC)for the repalr of skin defects in SD rat. Methods We identified BMSCs by flow cytometry after the bone marrow cells of SD rats were cultured,proliferated,purified,passaged,and then we inoculated BMSCs into ADM. After two days,the ADM combined with BMSC was transplanted into the skin defect of SD rats to observe the repalring effect for five weeks. Results The repalring effect of ADM combined with BMSC in the skin defect of SD rats was good. The visible material was linked closely and flexibly with the organization of SD rats,and the color was closed to the normal skin after five weeks. Conclusions ADM combined with BMSC could be used as a tissue engineering material for repalring the skin defect of SD rats.