CAJ | 학술논문

目的：建立测定金宝肾片中卡托普利含量的分析方法。方法采用高效液相色谱法，色谱柱为Lichrospher C18（4.6 mm×250 mm，5μm）柱，流动相为甲醇-四氢呋喃-0.1%磷酸溶液（30：0.15：70，pH=2.6~2.8），检测波长为215 nm，柱温为40℃；流速为1.0 mL/min；进样量为20μL。结果卡托普利浓度在18.964~94.820μg/mL（r=0.9998）范围内与峰面积线性关系良好，平均加样回收率为96.97%，RSD=0.60%（n=9）。结论本测定方法操作简单、准确，重复性好，可用于该制剂的质量控制。
목적：건립측정금보신편중잡탁보리함량적분석방법。방법채용고효액상색보법，색보주위Lichrospher C18（4.6 mm×250 mm，5μm）주，류동상위갑순-사경부남-0.1%린산용액（30：0.15：70，pH=2.6~2.8），검측파장위215 nm，주온위40℃；류속위1.0 mL/min；진양량위20μL。결과잡탁보리농도재18.964~94.820μg/mL（r=0.9998）범위내여봉면적선성관계량호，평균가양회수솔위96.97%，RSD=0.60%（n=9）。결론본측정방법조작간단、준학，중복성호，가용우해제제적질량공제。
Objective To establish a HPLC method for the determination of Captopril in Jinbaoshen Tablets. Methods HPLC was performed on Lichrospher C18 column (4.6 mmí250 mm, 5μm) and the mobile phase was consisted of methanol-tetrahydrofuran-0.1% phosphoric acid aqueous solution (30:0.15:70, pH=2.6-2.8). The flow rate was 1.0 mL/min, the wavelength of detection was 215 nm and the column temperature was 40℃. Results The linear relationship was good for captopril within the range of 18.964-94.820 μg/mL (r=0.9998) and the average recovery was 96.97%,RSD=0.60%(n=9). Conclusion The HPLC method is simple and practical with good reproducibility, which is suitable for the analysis and quality control of captopril in Jinbaoshen Tablets.