CAJ | 학술논문

目的：建立中药木瓜药材中总糖及还原糖的测定方法并对其含量进行测定，由此计算推测出药材中多糖含量。方法采用苯酚-浓硫酸法测定总糖含量，采用3，5-二硝基水杨酸法（DNS法）测定还原糖的含量。结果木瓜总糖含量为12.06%。DNS法测定还原糖的最佳条件为院测定波长560 nm，DNS最佳用量为5.0 mL，沸水浴加热5 min。测得药材中还原糖含量为11.58%。木瓜原药材中多糖含量为2.51%。结论苯酚浓硫酸法及DNS法可用于总糖及还原糖含量的测定，总糖和还原糖含量的高低在一定程度上可以反映多糖含量的多少。
목적：건립중약목과약재중총당급환원당적측정방법병대기함량진행측정，유차계산추측출약재중다당함량。방법채용분분-농류산법측정총당함량，채용3，5-이초기수양산법（DNS법）측정환원당적함량。결과목과총당함량위12.06%。DNS법측정환원당적최가조건위원측정파장560 nm，DNS최가용량위5.0 mL，비수욕가열5 min。측득약재중환원당함량위11.58%。목과원약재중다당함량위2.51%。결론분분농류산법급DNS법가용우총당급환원당함량적측정，총당화환원당함량적고저재일정정도상가이반영다당함량적다소。
Objective To establish a method for determination of total sugar and reducing sugar in Chaenomeles speciosa and to detect the content of sugar with the methods. Methods The content of total sugar was determined by phenol-sulfuric acid method. The concentration of reducing sugar was determined by 3, 5-dinitrosalicylic acid method. Results The content of total sugar in Chaenomeles speciosa was 12.06%. The optimum determination wavelength of DNS colorimetry was 560 nm. The proper volume of DNS sample was 5.0 mL. The best time for colour reaction in the condition of boiling water bath was kept for 5 min. The content of reducing sugar in Chaenomeles speciosa was 11.58%. The content of polysaccharides was 2.51%. Conclusion Phenol-sulfuric acid method and DNS method can be used for the determination of total sugar and reducing sugar. The content of total sugar and reducing sugar indicate the content of polysaccharides in some degree.