CAJ | 학술논문

采用平板稀释法从海南八门湾红树林潮间带、木果榄和海莲红树根际土壤样品中分离和筛选小单孢菌科放线菌，对其分离方法进行了评价和比较，并通过16S rRNA基因测序探索了八门湾红树林小单孢菌科放线菌的多样性。采用4种预处理方法和5种分离培养基，经过排除重复菌株后共得到115株放线菌。16S rRNA基因测序结果显示，这些放线菌分布在5个科、9个属。其中小单孢菌科的菌株约占全部菌株的90%（105株），主要分布于3个属：Micromonospora（85%），Jishengella（9.5%）和Verrucosispora（5.5%），与其亲缘关系最近的菌株的相似性分布于98.5%~100%之间。
채용평판희석법종해남팔문만홍수림조간대、목과람화해련홍수근제토양양품중분리화사선소단포균과방선균，대기분리방법진행료평개화비교，병통과16S rRNA기인측서탐색료팔문만홍수림소단포균과방선균적다양성。채용4충예처리방법화5충분리배양기，경과배제중복균주후공득도115주방선균。16S rRNA기인측서결과현시，저사방선균분포재5개과、9개속。기중소단포균과적균주약점전부균주적90%（105주），주요분포우3개속：Micromonospora（85%），Jishengella（9.5%）화Verrucosispora（5.5%），여기친연관계최근적균주적상사성분포우98.5%~100%지간。
Micromonosporaceae actinomycetes were isolated and screened from soil samples collected from rhizospher-ic soil of Xylocarpus mekongensis and Bruguiera sexangula in Wenchang Bamen Bay of Hainan intertidal zone adopting dilution-plate method,and the isolation methods were valuated and compared,and the diversity of Micromonosporace-ae actinomycetes in Bamen Bay mangrove forest were investigated with 16S rRNA gene sequencing. Four pre-treatment approaches and five isolation media were used,a total of 115 actinomycetes strains were obtained after removing the repetitive strains. The 16S rRNA gene sequencing results showed that these 115 actinomycetes belonged to 5 families and 9 genera. Among them Micromonosporaceae strains accounted for 90% of the total(105 strains),and mainly dis-tributed in three genera:Micromonospora(85%),Jishengella(9. 5%),and Verrucosispora(5. 5%),and the simi-larities of the strain with the nearest osculation distributed from 98. 5% to 100%.