CAJ | 학술논문

利用抗菌及卤虫致死活性模型，从中国南海海底沉积物来源的微生物中筛选到2株放线菌SCSIO WJ01和SCSIO ZJ63，其发酵产物具有较强活性，经16S rRNA基因序列分析这2株放线菌均为异壁放线菌Ac-tinoalloteichus sp.。HPLC-DAD分析显示2株放线菌能产生同一个主要的次级代谢产物，通过正相硅胶柱色谱、反相中压柱色谱、半制备高效液相色谱等手段，从SCSIO WJ01的发酵产物中分离获得了该化合物，运用ESI-MS、1 H及13 C NMR波谱分析鉴定为浅蓝霉素A（ Caerulomycin A）。此外，还从SCSIO WJ01的发酵产物中分离鉴定了浅蓝霉素D。
이용항균급서충치사활성모형，종중국남해해저침적물래원적미생물중사선도2주방선균SCSIO WJ01화SCSIO ZJ63，기발효산물구유교강활성，경16S rRNA기인서렬분석저2주방선균균위이벽방선균Ac-tinoalloteichus sp.。HPLC-DAD분석현시2주방선균능산생동일개주요적차급대사산물，통과정상규효주색보、반상중압주색보、반제비고효액상색보등수단，종SCSIO WJ01적발효산물중분리획득료해화합물，운용ESI-MS、1 H급13 C NMR파보분석감정위천람매소A（ Caerulomycin A）。차외，환종SCSIO WJ01적발효산물중분리감정료천람매소D。
Two marine-originated actinobacteria strains SCSIO WJ01 and SCSIO ZJ63 isolated and screened from sed-iments collected in the bottom of South China Sea,using antibiotic and lethal activities model against brine shrimp ( Artemia salina),their fermentation broths possessed fairly strong activity. These two strains were identified as Acti-noalloteichus sp. through 16S rRNA gene sequence analysis. HPLC-DAD analysis disclosed that strains SCSIO WJ01 and SCSIO ZJ63 could produce the same major secondary metabolite. These fermentation products were isolated from the fermentation extract of strain SCSIO WJ01 by means of ortho-phase silica gel column chromatography and counter-phase mid-pressure column chromatography,and semi-preparative HPLC etc and identified to be caerulomycin A using ESI-MS,1 H and 13 C NMR spectroscopic analyses. In addition,caerulomycin D was also isolated and identified from fermentation product of strain SCSIO WJ01.