CAJ | 학술논문

以来自鹿粪便中1株芽孢杆菌为试验菌株，在分子水平上通过16S rDNA同源性序列分析、比较、鉴定，并通过形态观察、革兰氏染色、芽孢染色、生理生化实验，初步确定该菌株为枯草芽孢杆菌J-2。分析影响芽孢杆菌J-2产生抗真菌肽的主要因素，通过正交优化实验得出，芽孢杆菌J-2产生抗真菌肽的最佳发酵条件为500 mL的锥形瓶中加入150 mL初始pH为7.0的PDL（200）培养基，接入9%的液体菌种，在温度为37℃条件下发酵24 h。
이래자록분편중1주아포간균위시험균주，재분자수평상통과16S rDNA동원성서렬분석、비교、감정，병통과형태관찰、혁란씨염색、아포염색、생리생화실험，초보학정해균주위고초아포간균J-2。분석영향아포간균J-2산생항진균태적주요인소，통과정교우화실험득출，아포간균J-2산생항진균태적최가발효조건위500 mL적추형병중가입150 mL초시pH위7.0적PDL（200）배양기，접입9%적액체균충，재온도위37℃조건하발효24 h。
In this study, with a strain of bacillus from deer feces as the test strains, we identified it as Bacillus subtilis J-2 by morphology, gram stain, spore stain, physiological and biochemical experiments, and it is analyzed, compared and identified by 16S rDNA sequence at the molecular level. the major factors which impact Bacillus J-2 generating antifungal peptides is analyzed by orthogonal optimization experiments, the optimum fermentation conditions of Bacillus J-2 generating antifungal peptides is derived, in 500 mL erlenmeyer flask is added 150 mL initial pH 7.0 PDL (200) medium access 9%liquid spawn, at the temperature of 37℃for fermentation. 24 h.