CAJ | 학술논문

动态观察肺炎支原体（ Mycoplasma pneumoniae，Mp）感染鼠T淋巴细胞的数量及其亚群的变化，为正确评价T淋巴细胞在Mp感染中的作用提供理论依据。Mp滴鼻感染小鼠，在感染后不同时间点采集标本，流式细胞术检测感染鼠外周血中T淋巴细胞数量变化，ELISA测定脾细胞上清中TGF-β1、IL-10含量。结果表明，Mp感染不同时间点外周血中CD4+T淋巴细胞数量变化不明显或呈下降趋势。CD4+/CD8+比值下降。初次感染和再次感染的第3天、第7天，TGF-β1、IL-10表达水平升高。肺炎支原体感染对CD4+T淋巴细胞有一定抑制作用，外周血TGF-β1、IL-10的表达与脾细胞中CD4+T淋巴细胞数量变化成反比。提示TGF-β1可能参与了对Th1细胞的负调控作用。
동태관찰폐염지원체（ Mycoplasma pneumoniae，Mp）감염서T림파세포적수량급기아군적변화，위정학평개T림파세포재Mp감염중적작용제공이론의거。Mp적비감염소서，재감염후불동시간점채집표본，류식세포술검측감염서외주혈중T림파세포수량변화，ELISA측정비세포상청중TGF-β1、IL-10함량。결과표명，Mp감염불동시간점외주혈중CD4+T림파세포수량변화불명현혹정하강추세。CD4+/CD8+비치하강。초차감염화재차감염적제3천、제7천，TGF-β1、IL-10표체수평승고。폐염지원체감염대CD4+T림파세포유일정억제작용，외주혈TGF-β1、IL-10적표체여비세포중CD4+T림파세포수량변화성반비。제시TGF-β1가능삼여료대Th1세포적부조공작용。
Dynamic changes of T cells quantity and their subgroups in Mycoplasma pneumoniae( Mp)infected mice were observed to properly evaluate the role of T cells in Mp infection and to provide theoretical basis for Mp infection study. BALB/c mice were intranasally infected with Mp whole cells. Samples were collected at different time points after the infection. The changes of peripheral blood of T lymphocyte quantity of the infected mice was counted by flow cytometry. Cytokines of spleen cells supernatant were assessed by ELISA. CD4+T-lymphocyte count of the peripheral blood had no difference or assumed a declining drift at the different time points after the Mp infection. CD4+ / CD8+ratio was decreased. The levels of TGF-β1,IL-10 were increased on day 3 and 7 of initial infection and re-infection. Therefore,Mp infection had a certain inhibition against CD4+ T cells,and the expression of TGF-β1 and IL-10 was in inverse proportion to the change of CD4+ T cell count of spleen cells. These suggested that TGF-β1 may participate in the negative regulation function of Th1 cells.