CAJ | 학술논문

目的：探讨EphA7基因在牙髓的炎症反应和牙源性疼痛中的作用及意义。方法：收集健康牙髓、具有疼痛症状的牙髓炎牙髓和无疼痛症状的牙髓炎牙髓各18例，分别采用免疫组化染色和Western印迹法检测EphA7蛋白在不同状态牙髓组织中的表达水平。结果：免疫组化染色显示：EphA7在健康牙髓中仅阳性表达于血管内皮细胞和成牙本质细胞；而在炎症牙髓中，EphA7的表达显著升高，除阳性表达于上述细胞外，同时还阳性表达于成纤维细胞、炎症细胞以及神经纤维组织中。Western印迹法检测结果显示：与健康牙髓相比，牙髓炎牙髓中EphA7蛋白的表达水平显著增高（P＜0．05），具有疼痛症状的牙髓中EphA7蛋白的表达水平显著高于无疼痛症状的牙髓（P＜0．05）。结论：EphA7基因可能是反映牙髓组织炎症活动和疼痛状态的一个标记物。
목적：탐토EphA7기인재아수적염증반응화아원성동통중적작용급의의。방법：수집건강아수、구유동통증상적아수염아수화무동통증상적아수염아수각18례，분별채용면역조화염색화Western인적법검측EphA7단백재불동상태아수조직중적표체수평。결과：면역조화염색현시：EphA7재건강아수중부양성표체우혈관내피세포화성아본질세포；이재염증아수중，EphA7적표체현저승고，제양성표체우상술세포외，동시환양성표체우성섬유세포、염증세포이급신경섬유조직중。Western인적법검측결과현시：여건강아수상비，아수염아수중EphA7단백적표체수평현저증고（P＜0．05），구유동통증상적아수중EphA7단백적표체수평현저고우무동통증상적아수（P＜0．05）。결론：EphA7기인가능시반영아수조직염증활동화동통상태적일개표기물。
AIM:To investigate the role of EphA7 gene in pulp inflammation and odontogenic pain. METHODS:18 healthy controls,18 asymptomatic and 18 symptomatic irreversible pulpitis human dental pulps were included in the study.The protein expression of EphA7 was detected by immunohistochemistry and western blotting re-spectively.RESULTS:In healthy samples,immunohistochemical staining showed that positive EphA7 expression was only in vascular endothelial cells and odontoblasts.In the inflammatory pulps ,EphA7 expression increased significant-ly.In addition to vascular endothelial cells and odontoblasts,EphA7 positive staining was also detected in fibroblasts, inflammatory cells and nerve fiber tissues.Western blotting analysis showed that EphA7 expression increased signifi-cantly in pulpitis samples compared with healthy controls.In symptomatic irreversible pulpitis samples,EphA7 expres-sion was significantly higher than those in asymptomatic ones.CONCLUSION:EphA7 gene may be a marker reflec-ting inflammatory and pain state of human dental pulp.