CAJ | 학술논문

建立了山楂片中苏丹红的薄层色谱-紫外可见分光光度测定方法.实验条件：展开剂为正己烷：丙酮：乙酸乙酯=100：6：8，检测波长：苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ分别在474 nm，488 nm，503 nm，510 nm 检测.此方法中苏丹红Ⅰ-Ⅳ号标准工作曲线的线性方程分别为 AⅠ=0.0464C +0.0031，RⅠ=1；AⅡ=0.0568C +0.0081，RⅡ=0.9999；AⅢ=0.0507C +0.0033，RⅢ=1；AⅣ=0.0236C +0.0012；RⅣ=1.线性范围分别是1-60μg/ mL，最低检出限分别为0.02μg/ mL、0.02μg/ mL、0.04μg/ mL、0.06μg/ mL，苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ回收率平均值分别为97.17%，98.00%，97.67%，95.05%.本方法设备使用方便，操作简单，且精密度和灵敏度好，准确度高，是一种简便可行的方法.
건립료산사편중소단홍적박층색보-자외가견분광광도측정방법.실험조건：전개제위정기완：병동：을산을지=100：6：8，검측파장：소단홍Ⅰ、Ⅱ、Ⅲ、Ⅳ분별재474 nm，488 nm，503 nm，510 nm 검측.차방법중소단홍Ⅰ-Ⅳ호표준공작곡선적선성방정분별위 AⅠ=0.0464C +0.0031，RⅠ=1；AⅡ=0.0568C +0.0081，RⅡ=0.9999；AⅢ=0.0507C +0.0033，RⅢ=1；AⅣ=0.0236C +0.0012；RⅣ=1.선성범위분별시1-60μg/ mL，최저검출한분별위0.02μg/ mL、0.02μg/ mL、0.04μg/ mL、0.06μg/ mL，소단홍Ⅰ、Ⅱ、Ⅲ、Ⅳ회수솔평균치분별위97.17%，98.00%，97.67%，95.05%.본방법설비사용방편，조작간단，차정밀도화령민도호，준학도고，시일충간편가행적방법.
A novel method of detecting Sudan in Haw Flakes is established by using the thin layer chromatog-raphy and the ultraviolet visible spectrophotometry. The reagent is hexane:acetone:ethyl acetate = 100:6:8,the detection wave length of SudanⅠ - Ⅳ is 474 nm,488 nm,503 nm,510 nm,respectively. The linear equations of Solvent RedⅠ - Ⅳ are the AⅠ = 0. 0464C + 0. 0031,RⅠ = 1;AⅡ = 0. 0568C + 0. 0081,RⅡ = 0. 9999;AⅢ =0. 0507C + 0. 0033,RⅢ = 1;AⅣ = 0. 0236C + 0. 0012;RⅣ = 1,all the linear ranges are from 1 to 60 μg / mL,the lowest detection limits are 0. 02 μg / mL,0. 02 μg / mL,0. 04 μg / mL,0. 06 μg / mL. Finally recovery rates of Su-danⅠ - Ⅳ are determined,the average values are 97. 17% ,98. 00% ,97. 67% ,95. 05% . This method is a simple and feasible,which has the advantages of using the convenient equipment,simple operation,good preci-sion and sensitivity,and high accuracy.