CAJ | 학술논문

基于植物能够利用体内的碳酸酐酶来催化碳酸氢根离子生成二氧化碳和水作为底物进行光合作用的特性,采用两种δ13 CPDB值差值大于10‰的碳酸氢钠分别作为外源碳酸氢根离子的碳同位素标记物,通过室内双向水培诸葛菜和芥菜型油菜实验,分别向水培处理液里添加已知δ13 CPDB值的碳酸氢钠并培养24 h,利用同位素比值质谱(IRMS)技术,测定并计算了两个时间、两种环境下的大气二氧化碳稳定碳同位素日平均组成。结果表明：在环境1(不同浓度的 NaHCO 3处理液)下所得到的δCa值与添加到处理液中的碳酸氢根离子的浓度有关；在环境2(不同浓度的 PEG 处理液)下所得到的δCa值与添加到处理液中的 PEG 的浓度无关；两种环境下所测得的大气二氧化碳稳定碳同位素日平均组成δCa 值与实验中培养的植物种类无关,而与添加到培养液中碳酸氢根离子的浓度及植物的生长速率有关。数据重现性好,结果准确可靠,可以高精度的测定不同待测环境下大气二氧化碳稳定碳同位素比值,其可为以后监测不同时间、不同地点的大气二氧化碳碳同位素组成及来源提供非常有效的方法和信息。
기우식물능구이용체내적탄산항매래최화탄산경근리자생성이양화탄화수작위저물진행광합작용적특성,채용량충δ13 CPDB치차치대우10‰적탄산경납분별작위외원탄산경근리자적탄동위소표기물,통과실내쌍향수배제갈채화개채형유채실험,분별향수배처리액리첨가이지δ13 CPDB치적탄산경납병배양24 h,이용동위소비치질보(IRMS)기술,측정병계산료량개시간、량충배경하적대기이양화탄은정탄동위소일평균조성。결과표명：재배경1(불동농도적 NaHCO 3처리액)하소득도적δCa치여첨가도처리액중적탄산경근리자적농도유관；재배경2(불동농도적 PEG 처리액)하소득도적δCa치여첨가도처리액중적 PEG 적농도무관；량충배경하소측득적대기이양화탄은정탄동위소일평균조성δCa 치여실험중배양적식물충류무관,이여첨가도배양액중탄산경근리자적농도급식물적생장속솔유관。수거중현성호,결과준학가고,가이고정도적측정불동대측배경하대기이양화탄은정탄동위소비치,기가위이후감측불동시간、불동지점적대기이양화탄탄동위소조성급래원제공비상유효적방법화신식。
Based on the characteristics that plants can take advantage of carbonic anhydrase enzyme to catalyze the bi-carbonate ions into carbon dioxide and water,which can serve as substrates for photosynthesis,two sodium bicarbon-ate whoseδ1 3 CPDB value differences were greater than 10‰ were used as the carbon isotope labeling and to bidirection-al water culture Orychophragmus violaceus and Brassica juncea lasted for 24 h in this study,respectively.The dally mean stable carbon isotope compositions in two different times and environments were investigated and analyzed ac-cording to isotope ratio mass spectrometry (IRMS)technique.In particular,Environment 1 represented adding some sodium bicarbonate to solutions to get different concentrations of bicarbonate solution and Environment 2 represented adding some polyethylene glycol to solutions to get different concentrations of PEG solutions.These results showed thatδCa values in the Environment 1 were related to the concentrations of added exogenous sodium bicarbonate to so-lution,but these in the Environment 2 had no significant correlation with PEG concentration in the treatment solu-tion,these results in this study suggested that the dally mean stable carbon isotope composition of atmospheric carbon dioxide had no relationship with the cultured plant species,but was related to the concentration of exogenous bicar-bonate ion added to the culture solution and growth rate of the two plants.The data obtalned with good reproducibili-ty and reliability,accurately determined the stable carbon isotope composition of atmospheric carbon dioxide in test environments.This study confirmed that the method was a very powerful tool for monitoring the carbon isotope com-position and sources of atmospheric carbon dioxide in different times and places for further.